I'm new to next-gen sequencing, so please be gentle. I've just received a pair of Illumina FASTQ files from the sequencing facility and intend to map them to the hg19 reference genome. I first used the FASTQ Groomer utility to convert the reads into Sanger reads. However, when running Bowtie for Illumina on the resulting dataset under default settings, I received the following error:
An error occurred running this job: Error aligning sequence. requested number of bytes is more than a Python string can hold
Appreciate the help!
Weng Khong, LIM
Department of Genetics
University of Cambridge