Hi all,<div><br></div><div>I&#39;m new to next-gen sequencing, so please be gentle. I&#39;ve just received a pair of Illumina FASTQ files from the sequencing facility and intend to map them to the hg19 reference genome. I first used the FASTQ Groomer utility to convert the reads into Sanger reads. However, when running Bowtie for Illumina on the resulting dataset under default settings, I received the following error:</div>
<div><br></div><div><span class="Apple-style-span" style="font-family: &#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif; font-size: 12px; color: rgb(48, 48, 48); line-height: 14px; ">An error occurred running this job: <i>Error aligning sequence. requested number of bytes is more than a Python string can hold</i></span></div>
<div><font class="Apple-style-span" color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif" size="3"><span class="Apple-style-span" style="font-size: 12px; line-height: 14px;"><i><br></i></span></font></div>
<div><font class="Apple-style-span" color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif" size="3"><span class="Apple-style-span" style="font-size: 12px; line-height: 14px;">Can someone help point out my mistake? My history is accessible at <a href="http://main.g2.bx.psu.edu/u/wengkhong_lim/h/chip-seq-pilot-batch">http://main.g2.bx.psu.edu/u/wengkhong_lim/h/chip-seq-pilot-batch</a></span></font></div>
<div><font class="Apple-style-span" color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif" size="3"><span class="Apple-style-span" style="font-size: 12px; line-height: 14px;"><br></span></font></div>
<div><font class="Apple-style-span" color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif" size="3"><span class="Apple-style-span" style="font-size: 12px; line-height: 14px;">Appreciate the help!</span></font></div>
<div><font class="Apple-style-span" color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif" size="3"><span class="Apple-style-span" style="font-size: 12px; line-height: 14px;"><br></span></font></div>
<div><font class="Apple-style-span" color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif" size="3"><span class="Apple-style-span" style="font-size: 12px; line-height: 14px;">Weng Khong, LIM</span></font></div>
<div><font class="Apple-style-span" color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif" size="3"><span class="Apple-style-span" style="font-size: 12px; line-height: 14px;">Department of Genetics<br>
University of Cambridge<br>E-mail: <a href="mailto:wkl24@cam.ac.uk">wkl24@cam.ac.uk</a> <br>Tel: +447503225832</span></font></div>