Hi Wang,

please check if your fastq file is associated with the correct fastq
format, fastqsanger probably.

Cheers,
Bjoern

> Hi,
>
> I just notice that NGS FASTQ Trimmer by column can't detect the fastq
> file I loaded. Anyone knows why. thanks a lot.
>
>
>
> On Fri, Jan 24, 2014 at 11:00 AM,
> <galaxy-user-request@lists.bx.psu.edu> wrote:
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>         Today's Topics:
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>            1. Re: Creating a Trackster visualisation from a
>         reference in
>               your history (Jeremy Goecks)
>
>
>         ----------------------------------------------------------------------
>
>         Message: 1
>         Date: Thu, 23 Jan 2014 15:49:44 -0500
>         From: Jeremy Goecks <jgoecks@email.gwu.edu>
>         To: graham etherington (TSL)
>                 <graham.etherington@sainsbury-laboratory.ac.uk>
>         Cc: galaxy-user@lists.bx.psu.edu
>         Subject: Re: [galaxy-user] Creating a Trackster visualisation
>         from a
>                 reference in your history
>         Message-ID:
>         <C3382F38-D243-4979-B6A7-BA13EF161EE5@email.gwu.edu>
>         Content-Type: text/plain; charset="windows-1252"
>
>         > Is it possible to create a custom build and use it to view a
>         SAM file without adding the .len and .2bit files in to the
>         Galaxy file system as an administrator?
>
>         Yes, it definitely is.
>
>         > If so, what am I doing wrong?
>
>
>         This is a Galaxy bug which has been fixed in this commit:
>
>         https://bitbucket.org/galaxy/galaxy-central/commits/117fef56513fc563dd231516196cfd601c1635e2
>
>         We have a release coming up, so this fix will be included in
>         the release and will make it to our public server soon. In the
>         meantime, note that you can use the genome fasta file rather
>         than the len file to create a custom build and everything
>         should work.
>
>         Thanks,
>         J.
>
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>         End of galaxy-user Digest, Vol 91, Issue 21
>         *******************************************
>
>
>
> --
> Gang Wang
> Ph.D. student
> Veterinary Integrative Bioscience
> College of Veterinary Medicine & Biomedical Sciences
> Texas A&M University
> College Station, TX  77843-4458
>
>
>
>
>
>
>
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