Hi,<br>    I have HiSeq2000 paired end sequence data in two separate FASTQ files. I need to filter the low quality scored sequences from my data to have a good assembly. So I decided to join the PE reads and then filter the low quality sequences in Galaxy. <br>


   To do this first I groomed the data using FASTQ groomer where I kept &quot;Sanger&quot; as Input FASTQ quality scores type. Then I tried to join the PE sequences using FASTQ joiner. However the FASTQ joiner did not join the PE sequences but only shown the failure Info as follows <div>


                <b><a><span>FASTQ joiner on data 8 and data 9</span></a></b><br>0 bytes<br>
                format:  <span>fastqsanger</span>, 
                database: 
                    <a href="https://main.g2.bx.psu.edu/datasets/d08dd42f0e2ed22b/edit" target="_blank">?</a>
            </div>
                Info: There were 4000000 known sequence reads not utilized. <br>Joined 0 of 4000000 read pairs (0.00%).<br><br>I am a new user and I have no idea where I am going wrong. Please suggest me how to overcome this problem. <br>
<br>Thanks.<br>

<br><br>-- <br><div style="color:rgb(0,0,153)"><span style="color:rgb(102,0,0)">******************************************************************************************************************</span></div>
<div style="display:inline"></div><br>