I fixed it, Thanks.
It was because I aligned to different genomes in BWA and GATK steps


2012/4/3 Lilach Friedman <lilachfr@gmail.com>
Hi Galaxy Team,
I'm trying to use GATK DepthOfCoverage, and to give a BAM file of alined results as input.


My steps were:
1. BWA alignment
2. SAM-to-BAM
3. rmdup.

when I'm trying to give 3 (rmdup results) as an input to GATK DepthOfCoverage, an error appears: "
 Sequences are not currently available for the specified build."

What did I do wrong?


Many thanks!
   Lilach