Hello, Use "Get Data -> Upload" or load your data into Galaxy with FTP (if large): http://galaxyproject.org/wiki/Learn/Upload%20via%20FTP Once the data is in Galaxy, this tutorial covers the general analysis path for RNA-seq data: http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise http://main.g2.bx.psu.edu/u/jeremy/p/transcriptome-analysis-faq Hopefully this helps to get you started, Best, Jen Galaxy team On 10/18/11 10:24 PM, santhi ramachandran wrote:
Hi,
I am having a fastq file for my RNA data analysis in my computer. How to make it available for RNA seq data analysis using tophat.
Sandy ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other Galaxy lists, please use the interface at:
-- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support