thanks for the information Ross but I'm lost with the guide for the fastqc tool. I don't understand how to resolve "missing tool dependecies" Date: Mon, 9 Dec 2013 22:30:58 +1100 Subject: Re: [galaxy-user] Problem with executable file in FastQC From: ross.lazarus@gmail.com To: braun_bio@hotmail.com CC: galaxy-user@lists.bx.psu.edu Hi, Jorge,Galaxy source includes the Galaxy interfaces but not the third party executables for tools like fastqc or bwa. They can be automagically installed if you install the tool from a tool shed but at a guess, you are working on your desktop with the fastqc tool in a recent clone of galaxy? Unfortunately, the tool can't run until you have the fastqc software working in a particular way hinted at in the guide for the fastqc tool on the page at http://wiki.galaxyproject.org/Admin/Tools/Tool%20Dependencies - try that please? There are things to do to make your instance more reliable and stable too - eg http://wiki.galaxyproject.org/Admin/Config/Performance/ProductionServer On Mon, Dec 9, 2013 at 9:48 PM, Jorge Braun <braun_bio@hotmail.com> wrote: Hi mates!! I have a problem with Galaxy... FastQC doesn't run because the file FastQC.py cann't find executable file FastQC.xml. Almost, that file is in the same directory called rgenetics, (seeing Linux terminal) . someone can help me? thanks and have a nice day :)