Dear all,

 

I am a Phd student working on chicken genomics, with limited experience in the bio-informatics field. I performed an RNA-Seq experiment with single end 50 bp reads to find differential gene expressions between different groups. I have mapped this data with Tophat. Does anyone know how I can get the raw data count out of galaxy in a tab-delimited file? I would like to perform a statistical analysis in other software, without the RPKM normalization that cufflinks offers.  Thank you very much.

 

Have a nice day.

 

Yours Sincerely, Els

 

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Ir. Els Willems

KU Leuven

Department of Biosystems

Division Livestock - Nutrition - Quality

Laboratory of Livestock Physiology

Kasteelpark Arenberg 30 bus 2456

B - 3001 Heverlee

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