Hello: I apologize for bothering you but I am just beginning to work with mRNAseq data and it appears as though Galaxy will be quite useful but I suspect, even after looking some of the tutorials that I will be either making mistakes or just not have a good sense of things. I used "Fetch" off a new Mac computer to ftp 2 datasets yesterday whose sum total memory was over 26gb. It took 5 and one half hours to do that (my login is elwood.linney@duke.edu). This morning I had hoped to align this fastq data using your tools. When I identified the two files to put them in the right, history column of galaxy, it appears as though this transfer is taking a long time. Could you give me a time expectation of how long it might take to transfer say a 13gb file into the program if it has already been ftp'd to your site? The unspoken question above is "am I doing something wrong". Sincerely, but naively, Elwood Linney Professor of Molecular Genetics and Microbiology Duke University Medical Center