Hi Anthony, If the overall goal is to share a reference genome so that others can also use it in a local Galaxy instance (yours or theirs), you have two choices (with #2 being the simplest): 1 - share the indexes and .loc files using regular file sharing methods (outside of Galaxy), then have them install the reference genome in their instance as a built-in index. This shouldn't involve data libraries, although I guess you could probably technically use them. Test first. The issue with not being able to use the indexes directly from a data library is not a factor. If the data are indexes, they don't belong in a history/dataset, as these are set-up files. This wiki covers NGS data setup, in case you or they need help: http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup 2 - place just the .fasta version of the reference genome in a data library then set permissions for the target users. They would then be able to import the reference genome into a history as a dataset and use it as a "Custom reference genome" with tools. Or, they could download it and use it as a base to build their own indexes, loc files, etc. if running a different Galaxy instance. When using a custom reference genome, only a .fastsa file is needed, the indexes are generated at run time. This will utilize more resources in your instance. More about custom reference genomes: http://wiki.galaxyproject.org/Support#Custom_reference_genome Take care, Jen Galaxy team On 12/14/12 2:48 AM, Anthony Bretaudeau wrote:

Hi, I have a galaxy instance where I would like to make some private data available to a specific group of users. The data I would like to make available are some bowtie indexes, so each index is made of several files with the same prefix. How can I do this? I have tried to put these files in a data library, but each file is seen individually, so it is not usable in the bowtie tool. I could add it to the bowtie_indices.loc file, but I see no way to restrict the access to a specific group of user. Any help would be welcome ! Thanks Anthony ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:

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