Hi Jen, Thank you very much! Best, John From: Jennifer Jackson Sent: Friday, August 23, 2013 12:21 AM To: 师云 Cc: galaxy-user@lists.bx.psu.edu Subject: Re: [galaxy-user] How to get random region of 1000-bp window in the chromosome not overlapping specific [gtf] file Hello John, Use "Make windows" with the sliding window option (for example, offset =1), then use "Text Manipulation -> Select random lines". Best, Jen Galaxy team On 8/22/13 7:06 AM, 师云 wrote: Dear Galaxy develop team: As the subject said, I need to obtain random regions which are 1000-bb, and then filter these regions to remove regions overlapping a specific [gtf] file. So can I obtain random regions with Galaxy? I found (Regional Variation)->Make windows may help me, but the tool doesn't work at random. bests John ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ -- Jennifer Hillman-Jackson http://galaxyproject.org