Hi Rich, Sorry for the delay in reply. You can configure the display of custom tracks at UCSC by clicking on the custom track within the UCSC genome browser. You can also convert your wig file to a bigwig file (under Convert Formats tool menu), in order to speed up the display of this data. For help with configuring custom tracks that you have loaded into the UCSC Genome Browser, please contact the UCSC Genome Browser team. Please let us know if we can provide additional information. Thanks for using Galaxy, Dan On Jul 5, 2011, at 5:48 PM, Richard Mark White wrote:

Hi, this should be simple but it is not..forgive the newbie question. i am doing chip-seq. bowtie>sam filter for mapped reads>MACS. i want to create a wiggle file that displays in ucsc, but when i choose the "WIG" option on macs, and then try to show it in UCSC, it treats each line of the created WIG file as a separate track, and obviously does not show it as a graph. is there a wiki page somewhere that can give me the basics? or can someone point me in the right direction? thanks.

rich

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