Hi Jennifer, I already tried 2 weeks ago to upload my whole exome data by FTP, according to the instructions you sent below. The files are too big, it should take days for each file, and it always stops in the middle after 12 hours or so. So I gave up. If I use Galaxy on a cloud, I can work with the files on my computer, or I will still have to upload them by ftp? I guess I will still have to upload them, so it doesn't solve my problem? Thank you for the help! Lilach 2011/9/12 Jennifer Jackson <jen@bx.psu.edu>

Hello Lilach,

On 9/12/11 7:18 AM, Jennifer Jackson wrote:

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Hi, I am a new user of Galaxy. I want to analyze whole human exome data with Galaxy and cannot upload the files (10 Gb each) to the server.

If you need help uploading larger files, this prior Q/A has the details for FTP transfer: http://lists.bx.psu.edu/**pipermail/galaxy-user/2011-** September/003162.html<http://lists.bx.psu.edu/pipermail/galaxy-user/2011-September/003162.html>

Should I install Galaxy on a PC, and work in Unix environment, or there

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is a more comfortable way? If I should install it - what should be the computer properties, and where can I find the instructions for using it? I found the instructions for installing in Wiki, but didn't find the what computer is should be (RAM, HD, etc).

If you still decide to use your own instance, Galaxy itself should run on most platforms. However, there may be hardware dependencies for the 3rd party tools you intend to incorporate and use. http://galaxyproject.org/wiki/**Admin<http://galaxyproject.org/wiki/Admin>see "Tools"

A simplified alternative to hosting a local instance is the Cloudman Galaxy option, please see: http://galaxyproject.org/wiki/**Admin/Cloud<http://galaxyproject.org/wiki/Admin/Cloud> http://galaxyproject.org/wiki/**Big%20Picture/Choices<http://galaxyproject.org/wiki/Big%20Picture/Choices>

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Thanks, Lilach

Hopefully this helps. Going forward, please send all new questions directly "to" the mailing list and all replies with a "reply-all" to include the mailing list.

Best,

Jen Galaxy team

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Thank you very much for the big help! Lilach 2011/9/12 Peter Cock <p.j.a.cock@googlemail.com>

On Mon, Sep 12, 2011 at 5:20 PM, Lilach F <lilachfr@gmail.com> wrote:

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Hi Jennifer, I already tried 2 weeks ago to upload my whole exome data by FTP, according to the instructions you sent below. The files are too big, it should take days for each file, and it always stops in the middle after 12 hours or so. So I gave up.

If I use Galaxy on a cloud, I can work with the files on my computer, or I will still have to upload them by ftp? I guess I will still have to upload them, so it doesn't solve my problem?

If you use Amazon, they have a service where you can post them a hard drive and they copy it onto their cloud for you. This costs money of course, but avoids the network problem.

The other option is to check with your local computing people - perhaps there is a cluster or local super computing group you can get access to?

Peter