loading fasq.gz files using FTP
Hi I am NEW to RNA-seq data and galaxy programs. When I try to up-load the RNA-seq data using FileZilla using my desktop PC, each file (about350 MB) takes about 1 hour to up-load. Realizing I have 4 samples, each sample has 9 fasq.gz files, each file is about 350 GB, I really need some advice how to make this up-load work. Thanks, Tao tpeng@fhcrc.org
Hello Tao, It seems there has been a misunderstanding. Originally in other communications, your data was explained to be "4 samples with 2.4 GB of seq data per sample". If your 4 samples are actually a set of files with a total size of 350GB each (350GB x 4), then combined this would exceed the maximum quota of 50G per file/200G per history at the public Galaxy instance at http://usegalaxy.org. The option for this much data is to run either a local or cloud install. Help can be found at: http://getgalaxy.org http://galaxyproject.org/Admin/Cloud Questions related to local or cloud installations can be sent to the galaxy-dev@bx.psu.edu mailing list. Other users on this list may also have advice to offer about working with very large datasets. Best wishes for your project, Jen Galaxy team On 8/9/11 2:42 PM, Peng, Tao wrote:
Hi I am NEW to RNA-seq data and galaxy programs. When I try to up-load the RNA-seq data using FileZilla using my desktop PC, each file (about350 MB) takes about 1 hour to up-load.
Realizing I have 4 samples, each sample has 9 fasq.gz files, each file is about 350 GB, I really need some advice how to make this up-load work.
Thanks,
Tao
tpeng@fhcrc.org
___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other Galaxy lists, please use the interface at:
-- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/Support
Hi jen, have 4 biological samples, the RNA-seq data for each sample have 8 to 9 fasq.gz files with each file about 350 MB. So this is within the limit by public galaxy. Tao -----Original Message----- From: Jennifer Jackson [mailto:jen@bx.psu.edu] Sent: Tuesday, August 09, 2011 4:03 PM To: Peng, Tao Cc: galaxy-user@bx.psu.edu Subject: Re: [galaxy-user] loading fasq.gz files using FTP Hello Tao, It seems there has been a misunderstanding. Originally in other communications, your data was explained to be "4 samples with 2.4 GB of seq data per sample". If your 4 samples are actually a set of files with a total size of 350GB each (350GB x 4), then combined this would exceed the maximum quota of 50G per file/200G per history at the public Galaxy instance at http://usegalaxy.org. The option for this much data is to run either a local or cloud install. Help can be found at: http://getgalaxy.org http://galaxyproject.org/Admin/Cloud Questions related to local or cloud installations can be sent to the galaxy-dev@bx.psu.edu mailing list. Other users on this list may also have advice to offer about working with very large datasets. Best wishes for your project, Jen Galaxy team On 8/9/11 2:42 PM, Peng, Tao wrote:
Hi I am NEW to RNA-seq data and galaxy programs. When I try to up-load the RNA-seq data using FileZilla using my desktop PC, each file (about350 MB) takes about 1 hour to up-load.
Realizing I have 4 samples, each sample has 9 fasq.gz files, each file is about 350 GB, I really need some advice how to make this up-load work.
Thanks,
Tao
tpeng@fhcrc.org
___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other Galaxy lists, please use the interface at:
-- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/Support
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Peng, Tao