Hi All, I got a bit disappointed when I failed in the following operation which seems to be simple. I uploaded mouse annotation/reference gene sets (GTF format) to Galaxy. The file is located at ftp://ftp.ensembl.org/pub/release-65/gtf/mus_musculus/. As far as I know, GTF file is tab delimited, thus I used "Filter and Sort" --"filter" With following condition:C1 == 19 . column 1 is where chromosomes are located. I want to pick up all lines which belong to chromosome19. However, I only got an empty file. I know this is not a bit issue to discuss here. But I still hope you can help me figure out what 's wrong. Thanks Jiwen ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/