I WANT TO ALIGNMENT MILLIONS OF SEQUENCES FROM A sRNA LIBRARY WITH A SMALL GENOME (2600 BASES). HOW CAN I DO THIS ALIGNMENT IN GALAXY?. I HAVE TRIED WITH MULTIPLE ALIGNMENTS FROM TOOLS, BUT I CAN NOT SELECT BOTH FILES. THANKS FOR YOUR HELP DIANA TREJO -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
Hello, For tools that allow a custom genome, the form will include an option to use a "Dataset from the history" (or similar). Then a sub-menu will pop up where the reference genome can be selected. Other basics: For the query sequences, Fastq or fasta format may be required. After upload, make certain the datatype is a fastq version and then run "Fastq Groomer". Most alignment tools require a groomed file. For the target genome, fasta format is required. After upload, this can be assigned if needed on the ""Edit Attributes" form (click on pencil icon). Hopefully this helps, Best, Jen Galaxy team On 10/6/11 8:57 AM, dtrejo@ira.cinvestav.mx wrote:
I WANT TO ALIGNMENT MILLIONS OF SEQUENCES FROM A sRNA LIBRARY WITH A SMALL GENOME (2600 BASES). HOW CAN I DO THIS ALIGNMENT IN GALAXY?. I HAVE TRIED WITH MULTIPLE ALIGNMENTS FROM TOOLS, BUT I CAN NOT SELECT BOTH FILES.
THANKS FOR YOUR HELP
DIANA TREJO
-- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/Support
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Jennifer Jackson