Dear All I am interested in using Galaxy to compare miRNA sequencing data. I have previously groomed, clipped and then mapped miRNA data onto hg19 using bowtie. I have then identified differentially expressed miRNAs using cuffdiff and the UCSC downloaded miRNA/snoRNA database. However, I have recently tried to repeat this process but find the cuffdiff is unable to run. I just wondered why cuffdiff is no longer working or if there is alternative approach to comparing miRNA expression? Best wishes Mark
Hello Mark, The public Main Galaxy instance has been very busy for the last week or so. The best strategy is to start the jobs that are the most important first, to leave these queued, and to not stop/restart jobs. These will eventually run in the order they were submitted with respect to other users also utilizing the public resource. If I have misunderstood your comments, please send more details. Best, Jen Galaxy team On 1/29/13 7:43 AM, Mark Lindsay wrote:
Dear All
I am interested in using Galaxy to compare miRNA sequencing data.
I have previously groomed, clipped and then mapped miRNA data onto hg19 using bowtie. I have then identified differentially expressed miRNAs using cuffdiff and the UCSC downloaded miRNA/snoRNA database.
However, I have recently tried to repeat this process but find the cuffdiff is unable to run. I just wondered why cuffdiff is no longer working or if there is alternative approach to comparing miRNA expression?
Best wishes
Mark
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participants (2)
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Jennifer Jackson
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Mark Lindsay