Dear Galaxy team, I am performing a differential expression analysis with a cufflinks-cuffmerge-cuffdiff workflow. I was wondering if the default value of 10 fragments for min alignment count is intended to exclude from the significance test all the loci under 10 counts across all samples or if the counts are 10 for at least one sample the test is performed? I thank you for your help Best regards davide --- Davide Degli Esposti, PhD Epigenetic (EGE) Group International Agency for Research on Cancer Tel. +33 4 72738036 Fax. +33 4 72738322 150, cours Albert Thomas 69372 Lyon Cedex 08 France ----------------------------------------------------------------------- This message and its attachments are strictly confidential. If you are not the intended recipient of this message, please immediately notify the sender and delete it. Since its integrity cannot be guaranteed, its content cannot involve the sender's responsibility. Any misuse, any disclosure or publication of its content, either whole or partial, is prohibited, exception made of formally approved use -----------------------------------------------------------------------
Hi Davide, I think it it is the first - making it so that both samples in any test would be over this minimum threshold. Best, Jen Galaxy team On 4/12/13 4:28 AM, Davide Degli Esposti wrote:
-- Jennifer Hillman-Jackson Galaxy Support and Training http://galaxyproject.org
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Davide Degli Esposti
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Jennifer Jackson