Hi Joachim, This is for your own local server? You can fix the byte-range issue issue by putting your Galaxy server behind a proxy such as nginx (http://wiki.g2.bx.psu.edu/Admin/Config/Performance/nginx%20Proxy) or apache (http://wiki.g2.bx.psu.edu/Admin/Config/Apache%20Proxy). It does look like Ensembl has changed their BAM handling and the links have stopped working, we'll work on a fix here, but do not have a time frame. Thanks, Dan On Sep 13, 2012, at 3:45 AM, Joachim Jacob wrote:

Hi all,

I have aligned RNA seq read with tophat to drosophila melanogaster 3 genome. However, I cannot view the alignment in UCSC (error Byte-range request was ignored by server), nor in Ensembl.

Error in Ensembl:

Malformed URL

The URL used to reach this page may be incomplete or out-of-date.

A location is required to build this page. For example, chromosomal coordinates:

http://www.ensembl.org/Drosophila_melanogaster/Location/View?r=2L:21650001-2...

Perhaps can somebody find out what I am doing wrong?

Thanks, Joachim

-- Joachim Jacob, PhD

Rijvisschestraat 120, 9052 Zwijnaarde Tel: +32 9 244.66.34 Bioinformatics Training and Services (BITS) http://www.bits.vib.be @bitsatvib

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