Hello Aleks, Without seeing your history, I guess would be that you need to assign a reference genome and then confirm that that reference genome is available at UCSC. If none of this seems to be the case with your issue, please send a shared history link and we can take a look. To share a link, from the right top of the history pane, use "Options" -> Share or Publish, create the share link, and email it back directly to me. Thanks! Jen Galaxy team On 7/12/11 3:29 PM, Aleks Schein wrote:

Hi all,

I am doing cufflinks assembly of TopHat-aligned NGS reads with Refseq gene track as reference annotation. All works, but resulting gtf files cannot be displayed in UCSC browser. I use "display at UCSC main" link, but get a message:"Error 500: Internal Server Error". Has anyone experienced this problem recently?

regards,

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