Hello Sonali, If the reference file is sequence and the little files are sequence, then using one of the NGS mapping tools would work (without the need to split/merge). This would make the assumption that "index position" means "positional coordinates" with respect to the reference. It is not exactly clear what you are trying to do, so if you would like to provide more information about the bio task you are trying to create a workflow for, we can offer more help. Perhaps there is another method to obtain the result you want. Best, Jen Galaxy team On 1/10/11 9:23 AM, Sonali Amonkar wrote:

Hi,

Am a complete newbie, at investigating Galaxy. I found my question(Scatter-Gather data) in the mailing list about 10 months ago, but did not see any action on it hence asking again.

I want to create a sub work flow where, 1. I scatter/split a file into little files, 2. Find the occurrence of each sequence in the little files, within a huge reference file and recreate the little files with the index positions appended against each sequence, as to where they appeared in the huge file. 3. Gather back the little files into a whole big file.

Can you tell me if it is possible to do this in Galaxy? If yes, are there any existing/in-built tools that I can use to do the same?

Thank you, Sonali Amonkar

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