Hello, Many tools that work with FASTQ inputs, including this one, require a dataset to be in ".fastqsanger" format. This wiki explains more about how to go from .fasta -> .fastasanger format (when necessary) and designating that format with the proper "datatype" assignment: http://wiki.galaxyproject.org/Support#Tool_doesn.27t_recognize_dataset See "Dataset special cases: FASTQ" Hopefully this helps, Jen Galaxy team On 5/16/13 2:31 PM, Nikolay N. wrote:

Hi,

I just started using the main galaxy instance. I uploaded via FTP and imported several FASTQ files. They all appear in the list of my "saved datasets" (see attached screenshot). However, when I click on any of the tools in the left panel (e.g. "FASTQ Summary Statistics") I get in the middle panel the info for the tool but the dropdown menu listing the available FASTQ files is empty (see attached screenshot). What do I need to do to be able to perform analysis on the FASTQ files that I uploaded?

many thanks,

Nikolay

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