Elwood: With large fastq files your best option will be either using public server (which is having difficulties today) or running a local instance of Galaxy. We are very much aware that the public site is not behaving well. It will be back on-line today and in the coming weeks we will be switching underlying infrastructure brining much needed relief to all Galaxy users at once. The best venue for posting such questions is out galaxy-user mailing list (I CC'ed it here). Thank you and sorry for the main site troubles. anton On Tue, Sep 24, 2013 at 11:29 AM, Elwood Linney <ellinney@gmail.com> wrote:

Hello, Because of the problems with Galaxy online, I am looking into using Galaxy on the cloud but even with all the information it sometimes is confusing for individuals who wish to use existing Galaxy in a specific manner.

In your video of transferring datasets to the cloud you used URLs for relatively small files, but under normal circumstances with Galaxy online, this does not work for large files. I generally work with fastq files of about 16gb in size. Would I be able to transfer this size of file via URL to the cloud or would I have to use some other means (for galaxy online I use fetch)?

Rather that bother you with this question, is there a specific address that I should send this type of question to (like the user list--though it seems like there is so much disfunctional with Galaxy online that it takes a few weeks to get a repy from that)?

Elwood Linney Professor of Molecular Genetics and Microbiology Duke University Medical Center

-- Anton Nekrutenko Associate Professor Dept. of Biochemistry and Molecular Biology www.galaxyproject.org (814) 826-3051