Hi Daniel for 2) you may use the tools under NGS QC and manipulation FASTQ to FASTAhttp://main.g2.bx.psu.edu/tool_runner?tool_id=cshl_fastq_to_fastaconverter

followed by

Collapsehttp://main.g2.bx.psu.edu/tool_runner?tool_id=cshl_fastx_collapsersequences

On 19 September 2011 09:54, Kevin Lam kevin@aitbiotech.com wrote:

For 1) you may refer to Simulated Dataset of Solexa - SEQanswershttp://seqanswers.com/forums/showthread.php?t=806

Has anyone replied you for 2) ?

On 18 September 2011 21:12, Daniel Sher dsher@sci.haifa.ac.il wrote:

...

Hello,

I have two questions - I apologize if they are trivial..

1. I want to simulate the amount of Illumina sequencing needed to

sequence and assemble a known genome. Is there a way to randomly pick sequences of a specific length from a genome (either one available online or one I upload)? Something like "pick 100bp randomly (either strand), move 400-500bp forward and pick another 100bp?"

2. Is there a way to remove redundant sequences from a FASTA file without

losing the original sequence names (as happens with "collapse")?

Thanks

Daniel

--

Daniel Sher, PhD
Department of Marine Biology
Leon H. Charney School of Marine Sciences
University of Haifa, Mt. Carmel 31905, Haifa, Israel

Office +972-4-8240731
Lab    +972-4-8288961
email: dsher@sci.haifa.ac.il


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