Hello, The dataset may need to have the specific type of .fastq format be set (such as .fastqsanger) for it to be used with certain tools. More information is in this wiki: http://wiki.galaxyproject.org/Support#Tool_doesn.27t_recognize_dataset Hopefully this helps, Jen Galaxy team On 8/8/13 8:06 PM, Mark Edward Fabreag wrote:

Good day.

I'm trying to use Galaxy's FASTQ to FASTA converter. However, the fastq file I generated using SAMTOOLS, can't be recognized.

Hope you could help me with this problem.

Thank you..

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