window and merge workflow?
Hi, I have two datasets, both from the same cell type and same genome build. One data set is chip-seq, windowed at either 1MB or 100Kb. The other is from a tiled array, with probes placed ~2-3kb apart. I want to window the latter at either 100Kb or 1Mb (an averaging of the values will suffice), and then intersect the two data sets so that for each window I'll have two data points: the chip-seq and the tiled array values. It seems like this should be possible using Galaxy, but I'm struggling with what the workflow should be. Does anyone have a workflow, or suggestions? Thanks in advance, David
Hello Zod, You can window the second dataset with the tool "Regional Variation -> Make windows" (to split). Then merge/compare the two data sources using tools in "Operate on Genomic Intervals". Hopefully this helps to get you started, Jen Galaxy team On 10/6/10 3:32 PM, zod wrote:
Hi,
I have two datasets, both from the same cell type and same genome build. One data set is chip-seq, windowed at either 1MB or 100Kb. The other is from a tiled array, with probes placed ~2-3kb apart.
I want to window the latter at either 100Kb or 1Mb (an averaging of the values will suffice), and then intersect the two data sets so that for each window I'll have two data points: the chip-seq and the tiled array values.
It seems like this should be possible using Galaxy, but I'm struggling with what the workflow should be. Does anyone have a workflow, or suggestions?
Thanks in advance,
David _______________________________________________ galaxy-user mailing list galaxy-user@lists.bx.psu.edu http://lists.bx.psu.edu/listinfo/galaxy-user
-- Jennifer Jackson http://usegalaxy.org
participants (2)
-
Jennifer Jackson
-
zod