Great :) Don't forget to CC the mailing list in future ;) On Fri, Feb 25, 2011 at 4:46 PM, Amy Boddy <aboddy@med.wayne.edu> wrote:

Thank you, these articles helped!

On Thu, Feb 24, 2011 at 5:47 PM, Peter Cock <p.j.a.cock@googlemail.com> wrote:

...

On Thu, Feb 24, 2011 at 8:47 PM, Amy Boddy <aboddy@med.wayne.edu> wrote:

...

Hello, I was wondering how Galaxy calculates the quality score for NGS data? Is there any documentation I could read on how this is calculated? Thanks! -Amy Boddy

Which quality score are you talking about? Raw reads? Mappings? If it's FASTQ files you mean, may I suggest:

http://dx.doi.org/10.1093/nar/gkp1137

and

http://en.wikipedia.org/wiki/FASTQ_format

Peter

-- Amy Boddy Ph.D Candidate Center for Molecular Medicine & Genetics Wayne State University School of Medicine 540 E. Canfield, Detroit MI 48201 Website: http://homopan.wayne.edu/ Phone: 313.577.0086 email: aboddy@med.wayne.edu