You should be able to do in Biomart I think fetch seq shoudl also allow you to do the same. Vasu ________________________________ Subject: Fwd: [galaxy-user] Cufflinks transcript-extract ---------- Forwarded message ----------From: dongdong zhaoweiming <zhaoweiming1983@yahoo.com.cn>Date: Sat, Oct 29, 2011 at 10:38 PMSubject: [galaxy-user] Cufflinks transcript-extractTo: galaxy-user@bx.psu.edu Hi, I use cufflinks to assemble my RNA-Seq reads which produced a GTF file, such as follows:(1)chr1 Cufflinks transcript 6234876 6235400 1000 + chr1 Cufflinks exon 6234876 6235060 1000 + chr1 Cufflinks exon 6235239 6235400 1000 +   (2) chr1 Cufflinks transcript 6230065 6230437 1000 chr1 Cufflinks exon 6230065 6230437 1000   I want to extract transcrip sequence from the (1) and (2). But fetch sequence could only extract genomic sequence, it only fit for transcrit which had one exon,so are there any other tools to extract transcripts which had

=2 exons sequence and combine it?   Thanks a lot! weimin zhao ___________________________________________________________The Galaxy User list should be used for the discussion ofGalaxy analysis and other features on the public serverat usegalaxy.org.  Please keep all replies on the list byusing "reply all" in your mail client.  For discussion oflocal Galaxy instances and the Galaxy source code, pleaseuse the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-devTo manage your subscriptions to this and other Galaxy lists,please use the interface at: http://lists.bx.psu.edu/