Hi Nadege, I am forwarding your question to galaxy-user@bx.psu.edu which is the best place to send data questions, so that the entire team can provide input. We will get back to you as soon as possible. Thanks! Jen Galaxy team ------- Hi Jen, I am completely new to the field of ChIP-seq and have, what may seem to many, a very trivial problem. Assuming that I have 2 datasets that I want to compare after normalizing each to their respective inputs, and assuming that these 2 datasets have very different number of reads to start with, how can I first normalize each dataset for number of reads in Galaxy? is it something that users do? Thanks for your precious help, best Nadege ________________________________________