Hi Peter,
What is bad is converting FASTA without qualities to FASTQ with made up qualities, regardless of if this is sequence or color space. I am a newbie to this. I didn't know about this. I was under the impression that 'conversion is bad whether one has the qual files or not!' Thanks for the input.
Do you have the quality scores for your data, in a *.qual file normally. Yes, I do have the *.qual files with me. I've tried to convert my data into FASTQ on my local installation but I was unsuccessful.
I've used this tool under NGS:QC and manipulation, and used my *.qual & *.csfasta files as an input. [image: Inline image 1] The tool didn't give me any error, but there was nothing inside the data set that is generated. PFA for further info from the [image: Inline image 4] button. [image: Inline image 3] So, could you pls suggest me something on what to do about this ? Thanks, Nikhil.
