Hi,
I am trying to integrate a tool into galaxy. The tool runs in two parts - 1) Computes correlation and provides an ouput 'txt' file (Java) 2) Takes the previously ouput txt file and outputs a 'pdf' file. (Python)
I am having trouble with the second tool. If I provide a sample 'txt' file as input, it's working fine. But if I run it in galaxy, it is not working (for some reason it is not reading the 'txt' file from the previous output). Below is the code.
#!/usr/bin/python
import sys, os import numpy as np import matplotlib.pyplot as plt
input_file = sys.argv[1] output_file = sys.argv[2]
print 'Number of arguments:', len(sys.argv), 'arguments.' print 'Argument List:', str(sys.argv)
txt_in = input_file + '.txt' mydata = np.loadtxt(txt_in) plt.figure(1) plt.plot(mydata[:,0],mydata[:,1],label = "Feature"); plt.plot(mydata[:,0],mydata[:,2], label = "Variance"); plt.xlabel('Distance from feature(bp)'); plt.ylabel('Score'); plt.title(sys.argv[1]); plt.legend(loc="best") pdf_out = output_file + '.pdf' plt.savefig(pdf_out)
data = file(pdf_out, 'rb').read() fp = open(output_file, 'wb') fp.write(data) fp.close() os.remove(txt_in) os.remove(pdf_out)
Below is the xml code.
<tool id="archtex_massdata_extraction" name="Extract mass data"> <description> for the given BAM file </description>
<command> java -jar Extraction.jar $input_bam_file $ref_filename $ref_filetype $output1 </command> <command interpreter="python"> plot.py $output1 $out_file1
</command>
<inputs> <param name="input_bam_file" type="data" format="BAM" label="Input BAM file" help="Choose an input BAM file"/> <param name="ref_filename" type="data" format="gen,txt,gtf,bed" label="Reference/Coordination file" help="Choose a reference file"/> <param name="ref_filetype" type="select" label="Choose the reference file type"> <option value="custom">Custom</option> <option value="refgene">refGene</option> <option value="GFF">GFF</option> <option value="bed">BED</option> </param> </inputs> <outputs> <data name="output1" format="txt" /> <data name="out_file1" format="pdf" /> </outputs> </tool>
I am forcing the input to be 'tx't and the output to be 'pdf' in the python script. When I run the code, it is not showing any errors but it's not showing any output either. I am able to download the first output 'txt' file but there is no download button option in the right pane of galaxy for the second part.
Any help is appreciated!
Thanks, VJ.