Thanks for your answer. I figured that there is an option to run a workflow on multiple files, but I can't merge the outputs afterwardsl. I would like the workflow to return one final output.
But you gave me another idea. Can I somehow tell one workflow to run on other workflow output? If this can be done, I can run 100 different workflows with bowtie & statistics, each working on one fastq file, than run another workflow which gets 100 xls inputs and merge them to one.
On Tue, Feb 12, 2013 at 5:20 PM, Joachim Jacob |VIB| joachim.jacob@vib.bewrote:
Hi Hagai,
Actually, using a workflow, you are able to select multiple input files, and let the workflow run separately on all input files.
I would proceed by creating a data library for all your fastq files, which you can upload via FTP, or via a system directory. You can use a sample of your fastq files to create the steps in a history you want to perform, and extract a workflow out of it. Next, copy all fastq files from a data library in a new history, and run your workflow on the all input files.
I hope this helps you further, Joachim
Joachim Jacob
Rijvisschestraat 120, 9052 Zwijnaarde Tel: +32 9 244.66.34 Bioinformatics Training and Services (BITS) http://www.bits.vib.be @bitsatvib
On 02/12/2013 04:02 PM, Hagai Cohen wrote:
Hi, I'm looking for a preferred way of running Bowtie (or any other tool) on multiple input files and run statistics on the Bowtie output afterwards.
The input is a directory of files fastq1..fastq100 The bowtie output should be bed1...bed100 The statistics tool should run on bed1...bed100 and return xls1..xls100 Then I will write a tool which will get xls1..xls100 and merge them to one final output.
I searched for a smiliar cases, and I couldn't figure anyone which had this problem before. Can't use the parallelism tag, because what will be the input for each tool? it should be a fastq file not a directory of fastq files. Neither I would like to run each fastq file in a different workflow - creating a mess.
I thought only on two solutions:
- Implement new datatypes: bed_dir & fastq_dir and implements new tool
wrappers which will get a folder instead of a file. 2. merge the input files before sending to bowtie, and use parallelism tag to make them be splitted & merged again on each tool.
Does anyone has any better suggestion?
Thanks, Hagai
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