I have a question about sending a bed to Ensembl or bam to IGV through Galaxy. I have setup a galaxy on my own pccluster. Everything is fine. But when I want to display the bam file to IGV, I got the error message about error loading files. "Error loading http://xxx.xx.xxx/galaxy/display_application/xxxxxxxxxx/data/galaxy_xxxxx.ba m: An error occurred while accessing: http://xxx.xx.xxx/galaxy/display_application/xxxxxxxxxx/data/galaxy_xxxxx.ba m Read error; BinaryCode in readmode; streamed file (filename not available)" When I use the url to get the file directly, it can be successfully downloaded. I meet the same situation when I send the bed file to display at Ensembl. The tract will not be shown on Ensembl. When I want to send the bed manually through url, I also get the error message about loading files. "Unable to access file. Server response: 500 read timeout" Does anyone know how to solve the problem?? Thanks a lot!! Best Regards, Terry Yeh