Thanks for looking into this. I have unzipped all the .gz files so that the
directory contains all .chain files. The liftover.loc file has also been
modified to refer to all the .chain files instead, as such:
I still run into the same error if I try to run the tool in Galaxy, though
(hg19ToMm9 mapping currently not available). The liftover command still
works fine through the command line.
I have a feeling the error has to do with the issue with Galaxy's job
runner returning anything written to stderr as an error, mentioned here:
Seems like it was an issue back in 2010. Has this been rectified?
Again, thank you for your help,
On Wed, Jul 4, 2012 at 9:42 PM, Jennifer Jackson <jen(a)bx.psu.edu> wrote:
Most of this sounds correct. The only issue is with compressed files.
Galaxy uses uncompressed liftOver data at our site and this is how we
instruct that it be set up (see
Would you be able to run a test to see if uncompressing resolves the
issue? Uncompress one of the over.chain files, modify the .loc file so that
the name also reflects the uncompressed naming, then try to use the UI
version of liftOver with this uncompressed data. Please let us know how
this works out (cc-ing the list, so that others can learn of the confirmed
For now, there is no automated way to enter the .loc data entries. There
is some development activity going on right now to address data setup
(including liftOver), but nothing is ready yet to be shared. When complete,
any new tools will be announced (loudly - we know that they are highly
On 7/3/12 10:21 PM, Kwon SoonJae wrote:
I installed a local instance of galaxy and some tools using the command
line, and for some reason liftover will work in the command line but won't
work in galaxy. Here's what I've done so far:
- Downloaded liftover standalone executable from the tool-dependencies
page in the Gwiki.
- Changed permissions on liftover executable to make it usable in the
command prompt (terminal, macosx) -> otherwise I get permission denied.
- Copied executable over to /usr/local/bin likewise to use in command
prompt and in Galaxy.
- Modified liftover.loc to include all the conversion types and
directories of chain.gz files I downloaded to use with the tool (I believe
liftover can make use of both .chain and .chain.gz files).
After that I tried to run liftover in Galaxy and it didn't work, giving
me some error that said hg19ToMm9 mapping is currently not available. It
seems to work fine using the command line though.
Since my goal is to setup galaxy locally, I really want to get this
working correctly. Can anyone guide me through what's gone wrong?
Also, there must be a better way of installing all these tools than
manually downloading every single one and using the command line, surely? I
had to type out the format of liftover.loc file manually for around 130
chain files... I'm convinced there has to be a better way.
Thanks in advance,
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