Hi All, I'm having an error at this: *Dataset 18: Wig/BedGraph-to-bigWig on data 12* Tool execution generated the following error message: grep: writing output: Broken pipe grep: writing output: Broken pipe grep: writing output: Broken pipe grep: writing output: Broken pipe .. put: Broken pipe grep: writing output: Broken pipe grep: writing output: Broken pipe .. grep: writing output: Broken pipe grep: write error Error running wigToBigWig. The tool produced the following additional output: hashMustFindVal: '1' not found I searched and found this link: http://redmine.soe.ucsc.edu/forum/index.php?t=msg&goto=10745&S=2a335135e76cf9b7160c0e9d41353767 which says that there is a naming convention difference. I followed what he did and replaced chrom=1 to chrom=chr1 etc, now it goes further, but still dies with error: line 18020168 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276760 line 18020169 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276770 line 18020170 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276780 line 18020171 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276790 line 18020172 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276800 line 18020173 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276810 line 18020174 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276820 line 18020175 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276830 line 18020176 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276840 line 18020177 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276850 line 18020178 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276860 line 18020179 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276870 line 18020180 of /tmp/t3: chromosome chr1 has 195276750 bases, but item ends at 195276880 Unrecognized line 18020181 of /tmp/t3: variableStep chr10 span=10 Error running wigToBigWig. The command line is: wigToBigWig /tmp/t3 /home/bioinfoadmin/app/galaxy-dist/tool-data/shared/ucsc/chrom/ucsc_gg4.len /home/bioinfoadmin/app/galaxy-dist/database/files/000/dataset_769.dat -clip 2>&1 || echo "Error running wigToBigWig." >&2 Quite puzzled...wondering if anyone has seen it before and could give me a hand. :-) I'll really appreciate! Thanks, Rui