Hi Dannon, Thanks so much, your protocol was extremely helpful!
Thanks for looking into ENA SRA. In our experience, the ENA SRA is the easiest and most reliable way of importing public NGS data into Galaxy. The ENA SRA has an actual button to click that will import your data set of choice into Galaxy, while retaining the data set ID. It is much more straight forward (and reliable) than using any of the NCBI databases.
Thanks for your help with all of this, Dannon! Have a great night.
Cheers, Mo Heydarian
PhD candidate The Johns Hopkins School of Medicine Department of Biological Chemistry 725 Wolfe Street 402 Biophysics Baltimore, MD 21205
On Wed, Apr 2, 2014 at 6:21 PM, Dannon Baker dannon.baker@gmail.com wrote:
On Wed, Apr 2, 2014 at 6:03 PM, Mohammad Heydarian mheydar1@jhmi.eduwrote:
Hi Dannon, Thanks for this information. Will the ENA SRA button be enabled on the next update of Cloudman?
I'll check to make sure there wasn't a good reason to remove it (couldn't find one in my first pass, and it's available on main, so it should be in the cloud), but yes.
Is there a protocol (or help page) available for how to re-enable tools in
the 'tool_conf.xml'?
With everything headed to the toolshed, hopefully this is a thing of the past very soon. The only documentation I found on the wiki is here: https://wiki.galaxyproject.org/Admin/Tools/AddToolTutorial
In short, you'll need to:
- ssh to your cloud galaxy instance
- navigate to /mnt/galaxy/galaxy-app
- open tool_conf.xml in your favorite editor, and add the line below,
exactly, in the Get Data section (the first one, begins on line 3):
<tool file="data_source/ebi_sra.xml" />
- Close down ssh,
- In a web browser, go to the cloud admin panel and click restart for the
Galaxy service.
One of the great things about Galaxy Cloudman is that someone who doesn't know programming (myself and many other biologists and clinicians) can seamlessly use the platform. Unfortunately, when trivial issues arise where the solution is to insert a line or two of code in the universe of Galaxy Cloudman people like me are stymied and really don't even know where to start. I know it requires work at some level, but a tutorial on "how to find your 'tool_conf.xml'" (or "dummies guide to quick Cloudman fixes") would likely be helpful to many end users (including myself).
We definitely do try to make it as ready-to-go out of the box as possible, but that's a great idea. I'll try to document this better.
Thanks again, Dannon!
Anytime, thanks for your patience and for using Galaxy/Cloudman!