Dear all My attempts to visualise a vcf file in trackster fail. I can visualise the corresponding SAM/BAM files fine. I can visualise the same file on Galaxy main too, but not in my local install. I receive the following error message: "Trackster Error Input error: found interval with block-counts not matching starts/sizes on line. sort: write failed: standard output: Broken pipe sort: write error needLargeMem: trying to allocate 0 bytes (limit: 100000000000)" The vcf format is in version 4.1. I am creating it from the sorted BAM file with samtools mpileup in the most basic way. What am I missing? Thanks a lot for your help Ulf ************************************************************************** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of Public Health England, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses by Symantec.Cloud, but please re-sweep any attachments before opening or saving. http://www.gov.uk/PHE **************************************************************************