Hello again, 1/ I managed to find WolfPsort on github. I followed the readme file and it work with the command line but when I tried to run it on galaxy with the one included in the package tmhmm and signal from peterjc, it gave me an error: cant locate fastafmt/GetOptWarnHandler.pm I put in my .bashrc the path of the file. I tried to put it directly in the /bin directory but it didnt change anything. Any idea anyone? 2/in the python script I can see: Num_thread = thread_count(sys.argv[2], default=4) Does it means that it will use at the maximum 4 threads? Can I assume it will use 4 core in parallel? 3/I use blast + from the devteam is there any possibility to use more than 1 core? If yes where? Thanks ! M Olivier CLAUDE- PhD Student INSERM/UPMC UMRS ICAN 1166 Equipe 2 Faculté de médecine Pitié-Salpêtrière 91, bld de l'Hôpital - 3ème étage - Porte 305 75013 Paris