details: http://www.bx.psu.edu/hg/galaxy/rev/9d67ae5ecda7 changeset: 2872:9d67ae5ecda7 user: Ross Lazarus ross.lazarus@gmail.com date: Mon Oct 12 13:19:31 2009 -0400 description: real replacement for stoopid symlink
1 file(s) affected in this change:
lib/galaxy/datatypes/genetics.py
diffs (671 lines):
diff -r 75a488a0cbc9 -r 9d67ae5ecda7 lib/galaxy/datatypes/genetics.py --- a/lib/galaxy/datatypes/genetics.py Mon Oct 12 12:48:37 2009 -0400 +++ b/lib/galaxy/datatypes/genetics.py Mon Oct 12 13:19:31 2009 -0400 @@ -0,0 +1,667 @@ +""" +rgenetics datatypes +Use at your peril +Ross Lazarus +for the rgenetics and galaxy projects + +genome graphs datatypes derived from Interval datatypes +genome graphs datasets have a header row with appropriate columnames +The first column is always the marker - eg columname = rs, first row= rs12345 if the rows are snps +subsequent row values are all numeric ! Will fail if any non numeric (eg '+' or 'NA') values +ross lazarus for rgenetics +august 20 2007 +""" + +import logging, os, sys, time, tempfile, shutil, string, glob +import data +from galaxy import util +from cgi import escape +import urllib +from galaxy.web import url_for +from galaxy.datatypes import metadata +from galaxy.datatypes.metadata import MetadataElement +from galaxy.datatypes.data import Text +from galaxy.datatypes.tabular import Tabular +from galaxy.datatypes.images import Html + +gal_Log = logging.getLogger(__name__) + +class GenomeGraphs( Tabular ): + """Tab delimited data containing a marker id and any number of numeric values""" + + """Add metadata elements""" + MetadataElement( name="markerCol", default=1, desc="Marker ID column", param=metadata.ColumnParameter ) + MetadataElement( name="columns", default=3, desc="Number of columns", readonly=True ) + MetadataElement( name="column_types", default=[], desc="Column types", readonly=True, visible=False ) + file_ext = 'gg' + + def __init__(self, **kwd): + """Initialize gg datatype, by adding UCSC display apps""" + Tabular.__init__(self, **kwd) + self.add_display_app ( 'ucsc', 'Genome Graph', 'as_ucsc_display_file', 'ucsc_links' ) + + def set_peek( self, dataset ): + """Set the peek and blurb text""" + if not dataset.dataset.purged: + dataset.peek = data.get_file_peek( dataset.file_name ) + dataset.blurb = util.commaify( str( data.get_line_count( dataset.file_name ) ) ) + " rows" + else: + dataset.peek = 'file does not exist' + dataset.blurb = 'file purged from disk' + + def get_mime(self): + """Returns the mime type of the datatype""" + return 'application/vnd.msexcel' + + def get_estimated_display_viewport( self, dataset ): + """Return a chrom, start, stop tuple for viewing a file.""" + raise notImplemented + + def as_ucsc_display_file( self, dataset, **kwd ): + """Returns file""" + return file(dataset.file_name,'r') + + def ucsc_links( self, dataset, type, app, base_url ): + """ from the ever-helpful angie hinrichs angie@soe.ucsc.edu + a genome graphs call looks like this + http://genome.ucsc.edu/cgi-bin/hgGenome?clade=mammal&org=Human&db=hg... + &hgGenome_dataSetDescription=test&hgGenome_formatType=best%20guess&hgGenome_markerType=best%20guess + &hgGenome_columnLabels=best%20guess&hgGenome_maxVal=&hgGenome_labelVals= + &hgGenome_maxGapToFill=25000000&hgGenome_uploadFile=http://galaxy.esphealth.org/datasets/333/display/index + &hgGenome_doSubmitUpload=submit + Galaxy gives this for an interval file + http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg18&position=chr1:1-1000&... + http%3A%2F%2Fgalaxy.esphealth.org%2Fdisplay_as%3Fid%3D339%26display_app%3Ducsc + """ + ret_val = [] + ggtail = '&hgGenome_doSubmitUpload=submit' + if not dataset.dbkey: + dataset.dbkey = 'hg18' # punt! + ret_val = [] + ggtail = '&hgGenome_doSubmitUpload=submit' + if not dataset.dbkey: + dataset.dbkey = 'hg18' # punt! + if dataset.has_data: + for site_name, site_url in util.get_ucsc_by_build(dataset.dbkey): + if site_name in app.config.ucsc_display_sites: + site_url = site_url.replace('/hgTracks?','/hgGenome?') # for genome graphs + display_url = urllib.quote_plus( "%s/display_as?id=%i&display_app=%s" % (base_url, dataset.id, type) ) + sl = ["%sdb=%s" % (site_url,dataset.dbkey ),] + sl.append("&hgGenome_dataSetName=%s&hgGenome_dataSetDescription=%s" % (dataset.name, 'GalaxyGG_data')) + sl.append("&hgGenome_formatType=best%20guess&hgGenome_markerType=best%20guess") + sl.append("&hgGenome_columnLabels=first%20row&hgGenome_maxVal=&hgGenome_labelVals=") + sl.append("&hgGenome_maxGapToFill=25000000&hgGenome_uploadFile=") + s = urllib.quote_plus( ''.join(sl) ) + link = "%s%s%s" % (s, display_url, ggtail ) + ret_val.append( (site_name, link) ) + return ret_val + + + def validate( self, dataset ): + """Validate a gg file - all numeric after header row""" + errors = list() + infile = open(dataset.file_name, "r") + for i,row in enumerate(infile): #drop header + badvals = [] + if i > 0: + ll = row.strip().split('\t') + for j,x in enumerate(ll): + try: + x = float(x) + except: + badval.append('col%d:%s' % (j+1,x)) + if len(badvals) > 0: + errors.append('row %d, %s' % (' '.join(badvals))) + return errors + + def repair_methods( self, dataset ): + """Return options for removing errors along with a description""" + return [("lines","Remove erroneous lines")] + + def sniff(self,filename): + """ + """ + infile = open(dataset.file_name, "r") + header= infile.next() # header + badvals = [] + for i,row in enumerate(infile[:10]): # sample first 10 rows + ll = row.strip().split('\t') + for j,x in enumerate(ll[1:]): # ignore first identifier col + try: + x = float(x) + except: + badval.append('col%d:%s' % (j+1,x)) + if len(badvals) > 0: + return False + else: + return True + +class rgTabList(Tabular): + """ for sampleid and for featureid lists of exclusions or inclusions in the clean tool + featureid subsets on statistical criteria -> specialized display such as gg + """ + file_ext = "rgTList" + + + def __init__(self, **kwd): + """Initialize featurelistt datatype""" + Tabular.__init__( self, **kwd ) + self.column_names = [] + + def make_html_table( self, dataset, skipchars=[] ): + """Create HTML table, used for displaying peek""" + out = ['<table cellspacing="0" cellpadding="3">'] + comments = [] + try: + # Generate column header + out.append( '<tr>' ) + for i, name in enumerate( self.column_names ): + out.append( '<th>%s.%s</th>' % ( str( i+1 ), name ) ) + if dataset.metadata.columns - len( self.column_names ) > 0: + for i in range( len( self.column_names ), dataset.metadata.columns ): + out.append( '<th>%s</th>' % str( i+1 ) ) + out.append( '</tr>' ) + out.append( self.make_html_peek_rows( dataset, skipchars=skipchars ) ) + out.append( '</table>' ) + out = "".join( out ) + except Exception, exc: + out = "Can't create peek %s" % exc + return out + + +class rgSampleList(rgTabList): + """ for sampleid exclusions or inclusions in the clean tool + output from QC eg excess het, gender error, ibd pair member,eigen outlier,excess mendel errors,... + since they can be uploaded, should be flexible + but they are persistent at least + same infrastructure for expression? + """ + file_ext = "rgSList" + + def __init__(self, **kwd): + """Initialize samplelist datatype""" + rgTabList.__init__( self, **kwd ) + self.column_names[0] = 'FID' + self.column_names[1] = 'IID' + # this is what Plink wants as at 2009 + + def sniff(self,filename): + """ + """ + infile = open(dataset.file_name, "r") + header= infile.next() # header + if header[0] == 'FID' and header[1] == 'IID': + return True + else: + return False + +class rgFeatureList( rgTabList ): + """ for featureid lists of exclusions or inclusions in the clean tool + output from QC eg low maf, high missingness, bad hwe in controls, excess mendel errors,... + featureid subsets on statistical criteria -> specialized display such as gg + same infrastructure for expression? + """ + file_ext = "rgFList" + + def __init__(self, **kwd): + """Initialize featurelist datatype""" + rgTabList.__init__( self, **kwd ) + for i,s in enumerate(['#FeatureId', 'Chr', 'Genpos', 'Mappos']): + self.column_names[i] = s + + +class Rgenetics(Html): + """class to use for rgenetics""" + + MetadataElement( name="base_name", desc="base name for all transformed versions of this genetic dataset", default="rgenetics", + readonly=True, set_in_upload=True) + + composite_type = 'auto_primary_file' + allow_datatype_change = False + file_ext = 'rgenetics' + + + def missing_meta( self, dataset=None, **kwargs): + """Checks for empty meta values""" + for key, value in dataset.metadata.items(): + if not value: + return True + return False + + def generate_primary_file( self, dataset = None ): + rval = ['<html><head><title>Rgenetics Galaxy Composite Dataset </title></head><p/>'] + rval.append('<div>This composite dataset is composed of the following files:<p/><ul>') + for composite_name, composite_file in self.get_composite_files( dataset = dataset ).iteritems(): + opt_text = '' + if composite_file.optional: + opt_text = ' (optional)' + rval.append( '<li><a href="%s" type="application/binary">%s</a>%s' % ( composite_name, composite_name, opt_text ) ) + rval.append( '</ul></div></html>' ) + return "\n".join( rval ) + +class SNPMatrix(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="snpmatrix" + + def set_peek( self, dataset ): + if not dataset.dataset.purged: + dataset.peek = "Binary RGenetics file" + dataset.blurb = data.nice_size( dataset.get_size() ) + else: + dataset.peek = 'file does not exist' + dataset.blurb = 'file purged from disk' + + def sniff(self,filename): + """ need to check the file header hex code + """ + infile = open(dataset.file_name, "b") + head = infile.read(16) + head = [hex(x) for x in head] + if head <> '': + return False + else: + return True + + +class Lped(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="lped" + + def __init__( self, **kwd ): + Rgenetics.__init__(self, **kwd) + self.add_composite_file( '%s.ped', description = 'Pedigree File', substitute_name_with_metadata = 'base_name', is_binary = True ) + self.add_composite_file( '%s.map', description = 'Map File', substitute_name_with_metadata = 'base_name', is_binary = True ) + + +class Pphe(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="pphe" + + def __init__( self, **kwd ): + Rgenetics.__init__(self, **kwd) + self.add_composite_file( '%s.pphe', description = 'Plink Phenotype File', substitute_name_with_metadata = 'base_name' ) + + +class Lmap(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="lmap" + +class Fphe(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="fphe" + + def __init__( self, **kwd ): + Rgenetics.__init__(self, **kwd) + self.add_composite_file( '%s.fphe', description = 'FBAT Phenotype File', substitute_name_with_metadata = 'base_name' ) + +class Phe(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="phe" + + def __init__( self, **kwd ): + Rgenetics.__init__(self, **kwd) + self.add_composite_file( '%s.phe', description = 'Phenotype File', substitute_name_with_metadata = 'base_name' ) + + + +class Fped(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="fped" + + def __init__( self, **kwd ): + Rgenetics.__init__(self, **kwd) + self.add_composite_file( '%s.fped', description = 'FBAT format pedfile', substitute_name_with_metadata = 'base_name' ) + + +class Pbed(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="pbed" + + def __init__( self, **kwd ): + Rgenetics.__init__(self, **kwd) + self.add_composite_file( '%s.bim', substitute_name_with_metadata = 'base_name', is_binary = True ) + self.add_composite_file( '%s.bed', substitute_name_with_metadata = 'base_name', is_binary = True ) + self.add_composite_file( '%s.fam', substitute_name_with_metadata = 'base_name', is_binary = True ) + +class Eigenstratgeno(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="eigenstratgeno" + + def __init__( self, **kwd ): + Rgenetics.__init__(self, **kwd) + self.add_composite_file( '%s.eigenstratgeno', substitute_name_with_metadata = 'base_name', is_binary = True ) + self.add_composite_file( '%s.ind', substitute_name_with_metadata = 'base_name', is_binary = True ) + self.add_composite_file( '%s.map', substitute_name_with_metadata = 'base_name', is_binary = True ) + + + +class Eigenstratpca(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="eigenstratpca" + + def __init__( self, **kwd ): + Rgenetics.__init__(self, **kwd) + self.add_composite_file( '%s.eigenstratpca', description = 'Eigenstrat PCA file', substitute_name_with_metadata = 'base_name' ) + + +class Snptest(Rgenetics): + """fake class to distinguish different species of Rgenetics data collections + """ + file_ext="snptest" + + +class Pheno(Tabular): + """ + base class for pheno files + """ + file_ext = 'pheno' + + +class RexpBase( Html ): + """base class for BioC data structures in Galaxy + must be constructed with the pheno data in place since that + goes into the metadata for each instance + """ + MetadataElement( name="columns", default=0, desc="Number of columns", visible=True ) + MetadataElement( name="column_names", default=[], desc="Column names", visible=True ) + MetadataElement(name="pheCols",default=[],desc="Select list for potentially interesting variables",visible=True) + MetadataElement( name="base_name", + desc="base name for all transformed versions of this expression dataset", default='rexpression', set_in_upload=True) + MetadataElement( name="pheno_path", desc="Path to phenotype data for this experiment", default="rexpression.pheno", visible=True) + file_ext = 'rexpbase' + html_table = None + is_binary = True + composite_type = 'auto_primary_file' + allow_datatype_change = False + + + def __init__( self, **kwd ): + Html.__init__(self,**kwd) + self.add_composite_file( '%s.pheno', description = 'Phenodata tab text file', + substitute_name_with_metadata = 'base_name', is_binary=True) + + def generate_primary_file( self, dataset = None ): + """ This is called only at upload to write the html file + cannot rename the datasets here - they come with the default unfortunately + """ + return '<html><head></head><body>AutoGenerated Primary File for Composite Dataset</body></html>' + + def get_phecols(self, phenolist=[], maxConc=20): + """ + sept 2009: cannot use whitespace to split - make a more complex structure here + and adjust the methods that rely on this structure + return interesting phenotype column names for an rexpression eset or affybatch + to use in array subsetting and so on. Returns a data structure for a + dynamic Galaxy select parameter. + A column with only 1 value doesn't change, so is not interesting for + analysis. A column with a different value in every row is equivalent to a unique + identifier so is also not interesting for anova or limma analysis - both these + are removed after the concordance (count of unique terms) is constructed for each + column. Then a complication - each remaining pair of columns is tested for + redundancy - if two columns are always paired, then only one is needed :) + """ + for nrows,row in enumerate(phenolist): # construct concordance + if len(row.strip()) == 0: + break + row = row.strip().split('\t') + if nrows == 0: # set up from header + head = row + totcols = len(row) + concordance = [{} for x in head] # list of dicts + else: + for col,code in enumerate(row): # keep column order correct + if col >= totcols: + gal_Log.warning('### get_phecols error in pheno file - row %d col %d (%s) longer than header %s' % (nrows, col, row, head)) + else: + concordance[col].setdefault(code,0) # first one is zero + concordance[col][code] += 1 + useCols = [] + useConc = [] # columns of interest to keep + nrows = len(phenolist) + nrows -= 1 # drop head from count + for c,conc in enumerate(concordance): # c is column number + if (len(conc) > 1) and (len(conc) < min(nrows,maxConc)): # not all same and not all different!! + useConc.append(conc) # keep concordance + useCols.append(c) # keep column + nuse = len(useCols) + # now to check for pairs of concordant columns - drop one of these. + delme = [] + p = phenolist[1:] # drop header + plist = [x.strip().split('\t') for x in p] # list of lists + phe = [[x[i] for i in useCols] for x in plist if len(x) >= totcols] # strip unused data + for i in range(0,(nuse-1)): # for each interesting column + for j in range(i+1,nuse): + kdict = {} + for row in phe: # row is a list of lists + k = '%s%s' % (row[i],row[j]) # composite key + kdict[k] = k + if (len(kdict.keys()) == len(concordance[useCols[j]])): # i and j are always matched + delme.append(j) + delme = list(set(delme)) # remove dupes + listCol = [] + delme.sort() + delme.reverse() # must delete from far end! + for i in delme: + del useConc[i] # get rid of concordance + del useCols[i] # and usecols entry + for i,conc in enumerate(useConc): # these are all unique columns for the design matrix + ccounts = [(conc.get(code,0),code) for code in conc.keys()] # decorate + ccounts.sort() + cc = [(x[1],x[0]) for x in ccounts] # list of code count tuples + codeDetails = (head[useCols[i]],cc) # ('foo',[('a',3),('b',11),..]) + listCol.append(codeDetails) + if len(listCol) > 0: + res = listCol + # metadata.pheCols becomes [('bar;22,zot;113','foo'), ...] + else: + res = [('no usable phenotype columns found',[('?',0),]),] + return res + + + + def get_pheno(self,dataset): + """expects a .pheno file in the extra_files_dir - ugh + note that R is wierd and adds the row.name in + the header so the columns are all wrong - unless you tell it not to. + A file can be written as + write.table(file='foo.pheno',pData(foo),sep='\t',quote=F,row.names=F) + """ + p = file(dataset.metadata.pheno_path,'r').readlines() + if len(p) > 0: # should only need to fix an R pheno file once + head = p[0].strip().split('\t') + line1 = p[1].strip().split('\t') + if len(head) < len(line1): + head.insert(0,'ChipFileName') # fix R write.table b0rken-ness + p[0] = '\t'.join(head) + else: + p = [] + return '\n'.join(p) + + def set_peek( self, dataset ): + """expects a .pheno file in the extra_files_dir - ugh + note that R is wierd and does not include the row.name in + the header. why?""" + if not dataset.dataset.purged: + pp = os.path.join(dataset.extra_files_path,'%s.pheno' % dataset.metadata.base_name) + try: + p = file(pp,'r').readlines() + except: + p = ['##failed to find %s' % pp,] + gal_Log.debug('@@@rexpression set_peek, dataset.name=%s,\npp=%s,\np=%s' % (dataset.name,pp,p[:3])) + dataset.peek = ''.join(p[:5]) + dataset.blurb = 'Galaxy Rexpression composite file' + else: + dataset.peek = 'file does not exist\n' + dataset.blurb = 'file purged from disk' + + def get_peek( self, dataset ): + """expects a .pheno file in the extra_files_dir - ugh + """ + pp = os.path.join(dataset.extra_files_path,'%s.pheno' % dataset.metadata.base_name) + try: + p = file(pp,'r').readlines() + except: + p = ['##failed to find %s' % pp] + gal_Log.debug('@@@rexpression get_peek, dataset.file_name=%s,\npp=%s,\np=%s' % (dataset.file_name,pp,p[:3])) + return ''.join(p[:5]) + + def get_file_peek(self,filename): + """ + can't really peek at a filename - need the extra_files_path and such? + """ + h = '## rexpression get_file_peek: no file found' + try: + h = file(filename,'r').readlines() + except: + pass + return ''.join(h[:5]) + + def regenerate_primary_file(self,dataset): + """cannot do this until we are setting metadata + """ + bn = dataset.metadata.base_name + flist = os.listdir(dataset.extra_files_path) + rval = ['<html><head><title>Files for Composite Dataset %s</title></head><p/>Comprises the following files:<p/><ul>' % (bn)] + for i,fname in enumerate(flist): + sfname = os.path.split(fname)[-1] + rval.append( '<li><a href="%s">%s</a>' % ( sfname, sfname ) ) + rval.append( '</ul></html>' ) + gal_Log.debug('rexpression regenerate primary file, writing %s' % rval) + f = file(dataset.file_name,'w') + f.write("\n".join( rval )) + f.write('\n') + f.close() + + """Add metadata elements""" + def init_meta( self, dataset, copy_from=None ): + if copy_from: + dataset.metadata = copy_from.metadata + + def set_meta( self, dataset, **kwd ): + + """ + NOTE we apply the tabular machinary to the phenodata extracted + from a BioC eSet or affybatch. + + """ + try: + flist = os.listdir(dataset.extra_files_path) + except: + gal_Log.debug('@@@rexpression set_meta failed - no dataset?') + return + bn = None + for f in flist: + n = os.path.splitext(f)[0] + if not bn: + bn = n + dataset.metadata.base_name = bn + if not bn: + bn = '?' + pn = '%s.pheno' % (bn) + pp = os.path.join(dataset.extra_files_path,pn) + dataset.metadata.pheno_path=pp + try: + pf = file(pp,'r').readlines() # read the basename.phenodata in the extra_files_path + except: + pf = None + if pf: + h = pf[0].strip() + h = h.split('\t') # hope is header + h = [escape(x) for x in h] + dataset.metadata.column_names = h + dataset.metadata.columns = len(h) + dataset.peek = ''.join(pf[:5]) + else: + dataset.metadata.column_names = [] + dataset.metadata.columns = 0 + dataset.peek = 'No pheno file found' + if len(pf) > 1: + dataset.metadata.pheCols = self.get_phecols(phenolist=pf) + else: + dataset.metadata.pheCols = [('','No useable phenotypes found',False),] + #self.regenerate_primary_file(dataset) + if not dataset.info: + dataset.info = 'Galaxy Expression datatype object' + if not dataset.blurb: + dataset.blurb = 'R loadable BioC expression object for the Rexpression Galaxy toolkit' + gal_Log.debug('@@@rexpression set_meta on dsn=%s, pf=%s, peek=%s' % (dataset.file_name,''.join(pf[:5]),dataset.peek)) + return True + + def make_html_table( self, pp='nothing supplied from peek\n'): + """Create HTML table, used for displaying peek""" + out = ['<table cellspacing="0" cellpadding="3">',] + p = pp.split('\n') + try: + # Generate column header + for i,row in enumerate(p): + lrow = row.strip().split('\t') + if i == 0: + orow = ['<th>%s</th>' % escape(x) for x in lrow] + orow.insert(0,'<tr>') + orow.append('</tr>') + else: + orow = ['<td>%s</td>' % escape(x) for x in lrow] + orow.insert(0,'<tr>') + orow.append('</tr>') + out.append(''.join(orow)) + out.append( '</table>' ) + out = "\n".join( out ) + except Exception, exc: + out = "Can't create html table %s" % str( exc ) + return out + + def display_peek( self, dataset ): + """Returns formatted html of peek""" + out=self.make_html_table(dataset.peek) + return out + + def get_mime(self): + """Returns the mime type of the datatype""" + return 'text/html' + + +class Affybatch( RexpBase ): + """derived class for BioC data structures in Galaxy """ + + file_ext = "affybatch" + + def __init__( self, **kwd ): + RexpBase.__init__(self, **kwd) + self.add_composite_file( '%s.affybatch', description = 'AffyBatch R object saved to file', + substitute_name_with_metadata = 'base_name', is_binary=True ) + +class Eset( RexpBase ): + """derived class for BioC data structures in Galaxy """ + file_ext = "eset" + + def __init__( self, **kwd ): + RexpBase.__init__(self, **kwd) + self.add_composite_file( '%s.eset', description = 'ESet R object saved to file', + substitute_name_with_metadata = 'base_name', is_binary = True ) + + +class MAlist( RexpBase ): + """derived class for BioC data structures in Galaxy """ + file_ext = "malist" + + def __init__( self, **kwd ): + RexpBase.__init__(self, **kwd) + self.add_composite_file( '%s.malist', description = 'MAlist R object saved to file', + substitute_name_with_metadata = 'base_name', is_binary = True ) + + +if __name__ == '__main__': + import doctest, sys + doctest.testmod(sys.modules[__name__]) + +