Jonas, Cuffmerge and Cuffcompare use the sam_fa_indices.loc for sequence data; this is done for historical reasons. You can see this if you take a look at the wrapper code: <galaxy_home>/tools/ngs_rna/cuffmerge_wrapper.py <galaxy_home>/tools/ngs_rna/cuffcompare_wrapper.py Best, J. On Oct 19, 2012, at 6:10 AM, Jonas Grauholm wrote:
Greetings,
I’m trying to analyse a number of mouse RNA-seq samples on a local galaxy install. When trying to run cuffmerge and cuffcompare I get the following error.
No sequence data found for dbkey M_musculus_Ensembl_NCBIM37, so sequence data cannot be used.
The tool works fine with a human reference genome so im fairly confident I just fumbled a config file somewhere. The problem is I been having difficulty locating exactly what file defines what sequence file is being used?
I looked through all my config files but so far I been unable to locate the error, could anyone possibly point me in the right direction?
Best Regards
Jonas Grauholm AROS Applied Biotechnology A/S SciencePark Skejby Brendstrupgaardsvej 102 DK+8200 Aarhus N Phone: +45 70271170
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