We get the following error when trying to run MACS in our local installation of Galaxy: INFO @ Mon, 30 Apr 2012 09:02:14: # ARGUMENTS LIST: # name = MACS_in_Galaxy # ChIP-seq file = /proj/galaxy/000/dataset_552.dat # control file = None # effective genome size = 2.70e+09 # tag size = 36 # band width = 300 # model fold = 32 # pvalue cutoff = 1.00e-05 # Ranges for calculating regional lambda are : 1000,5000,10000 INFO @ Mon, 30 Apr 2012 09:02:14: #1 read tag files... INFO @ Mon, 30 Apr 2012 09:02:14: #1 read treatment tags... Traceback (most recent call last): File "/nas02/apps/python-2.6.5/bin/macs", line 238, in <module> main() File "/nas02/apps/python-2.6.5/bin/macs", line 53, in main (treat, control) = _load_tag_files_options (options) File "/nas02/apps/python-2.6.5/bin/macs", line 211, in _load_tag_files_options treat = load_tag_file(options.tfile,options.format) File "/nas02/apps/python-2.6.5/bin/macs", line 231, in load_tag_file raise Exception("Format \"%s\" cannot be recognized!" % (format)) Exception: Format "SAM" cannot be recognized! Does anyone know what causes this? We are using MACS-1.3.2. Mike Waldron