Hej all, I'm also facing another problem which might be related to my previous error, when I run Trinity: samtools: error while loading shared libraries: libbz2.so.1.0: cannot open shared object file: No such file or directory samtools: error while loading shared libraries: libbz2.so.1.0: cannot open shared object file: No such file or directory Error outputting data Error: Encountered internal Bowtie 2 exception (#1) Command: /opt/galaxy/production/_conda/envs/__trinity@2.4.0/bin/bowtie2-align-s --wrapper basic-0 --local -k 2 --threads 5 -f --score-min G,46,0 -x /opt/galaxy/production/galaxy-dist/database/jobs_directory/018/18987/working/trinity_out_dir/chrysalis/inchworm.K25.L25.fa.min100 /opt/galaxy/production/galaxy-dist/database/jobs_directory/018/18987/working/trinity_out_dir/both.fa (ERR): bowtie2-align exited with value 1 Error, cmd: bash -c " set -o pipefail;bowtie2 --local -k 2 --threads 5 -f --score-min G,46,0 -x /opt/galaxy/production/galaxy-dist/database/jobs_directory/018/18987/working/trinity_out_dir/chrysalis/inchworm.K25.L25.fa.min100 /opt/galaxy/production/galaxy-dist/database/jobs_directory/018/18987/working/trinity_out_dir/both.fa | samtools view -@ 5 -F4 -Sb - | samtools sort -m 107374182 -@ 5 -no - - > /opt/galaxy/production/galaxy-dist/database/jobs_directory/018/18987/working/trinity_out_dir/chrysalis/iworm.bowtie.nameSorted.bam" 2>tmp.54212.stderr died with ret 32512 at /opt/galaxy/production/_conda/envs/__trinity@2.4.0/opt/trinity-2.4.0/PerlLib/Pipeliner.pm line 166. Pipeliner::run(Pipeliner=HASH(0xd8f630)) called at /opt/galaxy/production/_conda/envs/__trinity@2.4.0/bin/Trinity line 1750 main::run_chrysalis("/opt/galaxy/production/galaxy-dist/database/jobs_directory/01"..., "/opt/galaxy/production/galaxy-dist/database/jobs_directory/01"..., 200, 500, "RF", "/opt/galaxy/production/galaxy-dist/database/jobs_directory/01"..., "/opt/galaxy/production/galaxy-dist/database/jobs_directory/01"...) called at /opt/galaxy/production/_conda/envs/__trinity@2.4.0/bin/Trinity line 1595 main::run_Trinity() called at /opt/galaxy/production/_conda/envs/__trinity@2.4.0/bin/Trinity line 1262 eval {...} called at /opt/galaxy/production/_conda/envs/__trinity@2.4.0/bin/Trinity line 1261 Trinity run failed. Must investigate error above. has anyone got an idea? Cheers Jochen