16 Jul
2012
16 Jul
'12
9:57 p.m.
Hi guys, I'm new wth NGS and have few basic questions about Galaxy for RNAseq: 1. when I run Cufflinks on my TopHat accepted hits, WHEN and WHY do i have to chose a reference annotation?Results are obviously different 2.if i chose this option, which one of the 2 references I have to upload from UCSC: RefSeq Genes or Knowngenes (both under Gene and genes prediction tracks).
Hope anyone can help
Thanks ib