Hello,
I have a question which I have not seen specifically addressed in the online Galaxy wiki documentation about how to integrate tools (dependencies) into Galaxy. I have implemented a locally managed instance of Galaxy that my business is using with our cluster and now have a freshly installed and configured instance of Galaxy running. It is bare-bones right now and I did not use mercurial to sync any existing files/directory structures. I have seen the page on external tool dependencies ( http://wiki.g2.bx.psu.edu/Admin/Tools/Tool%20Dependencies) needed for Galaxy, but I am somewhat unsure where to place the tools to utilize them as intended (other than through trial & error).
It appears that there are shell directories for the tools under ~/galaxy-dist/tools/ with basic wrapper scripts but without the corresponding executables (very few that I've noticed have the tools already in them). Is the intent to download the dependency tools and (building from source if necessary) take the binaries in those directories and copy them to their corresponding directory under ~/galaxy-dist/tools/? This seems to have worked with an error I first got when clipping a FASTQ file which reported that fastx_clipper was not a recognized command. So I downloaded the FASTX Toolkit, compiled the binaries, and copied only the binaries into the corresponding fastx tools directory. Would I do the same thing for TopHat and Cufflinks by taking all their binaries (combined) and copying them into ~/galaxy-dist/tools/ngs_rna/?
Even if that is the case though, I have occasionally gotten errors about tools missing in completely different directories. One was for the FASTQ Groomer. One user saw this error in their browser (which for now is the only way I know to figure out where tools are *expected* to be):
*File "/home/galaxy/galaxy-dist/tools/rgenetics/rgFastQC.py", line 141, in assert os.path.isfile(opts.executable),'##rgFastQC.py error - cannot find executable %s' % opts.executable AssertionError: ##rgFastQC.py error - cannot find executable /home/galaxy/galaxy-dist/tool-data/shared/jars/FastQC/fastqc *
To fix this I downloaded the FastQC tar file from its webpage, unzipped it, and copied the fastqc binary/script to the "home/galaxy/galaxy -dist/tool-data/shared/jars/FastQC/*"* directory. I also had to mkdir FastQC/ under jars/ to place it there since it didn't already exist. Had I not been told the specific directory by the error I'm not sure how I would have intuitively known to place the binary there (unless I'm overlooking some critical documentation). And how do I know that other similar things are not missing which should be there? Can anyone shed some light on this please? Adding a brief page on the Galaxy wiki site under the Admin section about this would really help, even if it only showed an example for one or two specific tools.
Thanks, Josh