Can you provide more details - in particular the version of Galaxy you are running? A step-by-step description of how you did the data import might be useful too for the Galaxy team to try to reproduce this. Peter On Fri, Mar 18, 2016 at 5:34 PM, Fabien Mareuil <fmareuil@pasteur.fr> wrote:
Hi Peter,
Thank a lot for this information, I didn't found this option for the new version of the Data Libraries. This could explain why there is this conversion. I tested it with an automatic format detection and when I set explicitly the format but in the two cases a tab was added.
Fabien
Le 18/03/2016 17:04, Peter Cock a écrit :
Hi Fabien,
There is a Galaxy upload option to turn spaces into tabs, useful for tables of data. Is that being ticked by mistake? That would explain this problem.
Also I would check if the FASTQ file format is being detected automatically, or set explicitly (e.g. fastqsanger)? Doing that ought to prevent any changes.
Peter
On Fri, Mar 18, 2016 at 3:26 PM, Fabien Mareuil <fmareuil@pasteur.fr> wrote:
Hi,
We got a problem with the new version of Data libraries. When we upload a fastq file from a User Directory, a tabulation character is added in the header in replacement of a space character.
from @HWI-D00395:188:HYGNFADXX:2:1108:1315:1965 1:N:0:ATGAGC to @HWI-D00395:188:HYGNFADXX:2:1108:1315:1965 1:N:0:ATGAGC
This behavior causes an error with cutadapt 1.6 (maybe with other tools) because it doesn't work with tab character. With the direct upload tool or the deprecated version of Data Libraries no tab character is added. Do you have some idea to correct this problem? Thanks a lot Best regards -- Fabien Mareuil | Centre d'Informatique pour la Biologie fabien.mareuil@pasteur.fr | Institut Pasteur 25,28 rue du Docteur Roux 75015 Paris, France
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