Hello, I've been trying to get working a local galaxy installation, using nginx with the upload module as a proxy. It all seems to work, except uploading files to Galaxy, where I get a "GET /_upload/ HTTP/1.1" 404 and nothing else Ic an find in the logs. There must be some misconfiguration somewhere, but I can't sport it. Could you help me? Here's my nginx.conf: user galaxy;

worker_processes 10; pid /run/nginx.pid;

events { worker_connections 768; } http { gzip on; gzip_http_version 1.1; gzip_vary on; gzip_comp_level 4; gzip_proxied any; gzip_types text/plain text/css application/x-javascript text/xml application/xml text/javascript application/json; gzip_buffers 16 8k; gzip_disable "MSIE [1-6].(?!.*SV1)"; upstream galaxy_app { server localhost:8080; } server { client_max_body_size 10G; location / { proxy_pass http://galaxy_app; proxy_set_header X-Forwarded-Host $host; proxy_set_header X-Forwarded-For $proxy_add_x_forwarded_for; } location /_x_accel_redirect/ { internal; alias /; } location ~ ^/plugins/visualizations/(?<vis_name>.+?)/static/(?<static_file>.*?)$ { alias /home/galaxy/galaxy/config/plugins/visualizations/$vis_name/static/$static_file;

expires 24h; } location /static { alias /home/galaxy/galaxy/static; expires 24h; } location /static/style { alias /home/galaxy/galaxy/static/june_2007_style/blue; expires 24h; } location /static/scripts { alias /home/galaxy/galaxy/static/scripts/packed; expires 24h; } location /favicon.ico { alias /home/galaxy/galaxy/static/favicon.ico; expires 24h; } location /robots.txt { alias /home/galaxy/galaxy/static/robots.txt; expires 24h; } location /_upload { upload_store /home/galaxy/galaxy/database/tmp/upload_store; upload_pass_form_field ""; upload_set_form_field "__${upload_field_name}__is_composite" "true"; upload_set_form_field "__${upload_field_name}__keys" "name path"; upload_set_form_field "${upload_field_name}_name" "$upload_file_name"; upload_set_form_field "${upload_field_name}_path" "$upload_tmp_path"; upload_pass_args on; upload_pass /_upload_done; } location /_upload_done { set $dst /api/tools; if ($args ~ nginx_redir=([^&]+)) { set $dst $1; } rewrite "" $dst; } location /plugins { alias /home/galaxy/galaxy-dist/config/plugins; } } }

And here's my galaxy.ini: [server:handler0]

port = 8090 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10

[server:handler1] port = 8091 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10

[server:handler2] port = 8092 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10

[server:handler3] port = 8093 host = 127.0.0.1 use_threadpool = true threadpool_workers = 10

[server:main] port = 8080 host = 0.0.0.0 use_threadpool = True threadpool_workers = 30 threadpool_kill_thread_limit = 10800

[filter:gzip]

[filter:proxy-prefix] prefix = /galaxy

[app:main] paste.app_factory = galaxy.web.buildapp:app_factory database_connection = postgresql://galaxy:not_the_actual_password@localhost /galaxy?host=/var/run/postgresql nginx_x_accel_redirect_base = /_x_accel_redirect nginx_upload_store = database/tmp/upload_store nginx_upload_path = /_upload tool_dependency_dir = dep_dir use_nglims = False nglims_config_file = tool-data/nglims.yaml static_enabled = True debug = False use_interactive = False id_secret = ValarDohaerys admin_users = adria.cereto@ctns.cat, galactus@galaxy.gal allow_user_impersonation = True allow_user_dataset_purge = True new_user_dataset_access_role_default_private = True expose_user_name = True master_api_key = heavysplatling track_jobs_in_database = True enable_job_recovery = True

[galaxy_amqp]

[uwsgi] processes = 44 stats = 127.0.0.1:9191 socket = 127.0.0.1:4001 pythonpath = lib threads = 12 logto = /home/galaxy/uwsgi.log master = True

Thank you in advance, Adrià -- *Adrià Cereto i Massagué* Centre for Omic Sciences (COS) www.omicscentre.com Servei de Recursos Científics i Tècnics (SRCiT) Universitat Rovira i Virgili Av. Universitat, 1. 43204 Reus (Catalonia) Tel: +34 977 77 69 32 <%2B34%20977%2077%2014%2009> Extensió URV: 4838 Languages: Català, Español, English, Français, Deutsch, Português Important Notice <http://www.gnu.org/philosophy/no-word-attachments.html>