Dear Sir I set up my local Galaxy with Apache proxy according to document https://wiki.galaxyproject.org/Admin/Config/ApacheProxy This work for my website only if I take the port number 8080 with my domian name. If I remove the port, it does not work. I am new to Galaxy, is there anyone can find out the problem of my settings? With port it is work [image: 内嵌图片 1] Without it does not work [image: 内嵌图片 2] My Apache configure file for galaxy is like this *conf.d/galaxy-dev.conf* [image: 内嵌图片 3] my ​universe_wsgi.ini​ configuration for port [image: 内嵌图片 4] ​[image: 内嵌图片 5]​ [image: 内嵌图片 6] Any suggestions are welcome, thanks in advance for your help. Regards Xiao-Tao Jiang -- Regards, Xiao-Tao Jiang Department of Engineering The University of HongKong