Pick-you-own columns in BLAST+ tabular output
Hello all, I've mentioned before that a forthcoming update to the BLAST+ Galaxy wrappers would be adding a most customisable output option with pick-your-own columns. This is available to test now from the Test Tool Shed, along with other enhancements like more masking tools and an update to BLAST+ 2.2.29: http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus (This will be released as the ncbi_blast_plus wrappers v0.1.0, and includes recent contributions from Jim Johnson and Björn Grüning - thank you both): Previously (for tabular output) we've only offered the standard 12 column output (-outfmt 6), and an extended set which has grown to currently include 25 columns. The new custom column mode offers the 12 standard columns, and the extended set (making 25 columns), plus other (to me less interesting) identifiers, some other miscellaneous columns, and the taxonomy columns (44 columns in all): [image: Inline image 1] [image: Inline image 2] As previously discussed, the list of columns has been grouped - the first two groups reflect the standard 12 columns and the columns used in Galaxy's (default) 25 column extended tabular output. The remaining columns I have split into three groups (assorted identifiers, miscellaneous, and taxonomy) which I hope is self explanatory. Note that this does *not* allow you pick the column order - they will be produced in the order shown. The underlying BLAST+ tools would support this, but the Galaxy GUI would I feel be too complicated. Some community testing/comments would be appreciated. Thanks, Peter
Hi Peter,
Hello all,
I've mentioned before that a forthcoming update to the BLAST+ Galaxy wrappers would be adding a most customisable output option with pick-your-own columns. This is available to test now from the Test Tool Shed, along with other enhancements like more masking tools and an update to BLAST+ 2.2.29:
http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus
(This will be released as the ncbi_blast_plus wrappers v0.1.0, and includes recent contributions from Jim Johnson and Björn Grüning - thank you both):
Previously (for tabular output) we've only offered the standard 12 column output (-outfmt 6), and an extended set which has grown to currently include 25 columns.
The new custom column mode offers the 12 standard columns, and the extended set (making 25 columns), plus other (to me less interesting) identifiers, some other miscellaneous columns, and the taxonomy columns (44 columns in all):
[image: Inline image 1] [image: Inline image 2]
As previously discussed, the list of columns has been grouped - the first two groups reflect the standard 12 columns and the columns used in Galaxy's (default) 25 column extended tabular output.
The remaining columns I have split into three groups (assorted identifiers, miscellaneous, and taxonomy) which I hope is self explanatory.
Note that this does *not* allow you pick the column order - they will be produced in the order shown. The underlying BLAST+ tools would support this, but the Galaxy GUI would I feel be too complicated.
I think that is fine, I can't imagine of any real use case where that is needed.
Some community testing/comments would be appreciated.
I tested it briefly on my test instance and it looks all fine and seems to work :) Thanks, looking forward to see it in MainTS. Bjoern
Thanks,
Peter
On Tue, Mar 4, 2014 at 10:35 AM, Björn Grüning <bjoern.gruening@gmail.com> wrote:
Hi Peter,
Hello all,
I've mentioned before that a forthcoming update to the BLAST+ Galaxy wrappers would be adding a most customisable output option with pick-your-own columns. This is available to test now from the Test Tool Shed, along with other enhancements like more masking tools and an update to BLAST+ 2.2.29:
http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus
...
Note that this does *not* allow you pick the column order - they
will be produced in the order shown. The underlying BLAST+ tools would support this, but the Galaxy GUI would I feel be too complicated.
I think that is fine, I can't imagine of any real use case where that is needed.
Some community testing/comments would be appreciated.
I tested it briefly on my test instance and it looks all fine and seems to work :)
Thanks, looking forward to see it in MainTS. Bjoern
Great - thanks. I'm about to update our local production server, and barring any problems will push this BLAST+ update to the main Tool Shed later this week. Thanks, Peter
On Tue, Mar 4, 2014 at 12:19 PM, Peter Cock <p.j.a.cock@googlemail.com> wrote:
On Tue, Mar 4, 2014 at 10:35 AM, Björn Grüning <bjoern.gruening@gmail.com> wrote:
Hi Peter,
Hello all,
I've mentioned before that a forthcoming update to the BLAST+ Galaxy wrappers would be adding a most customisable output option with pick-your-own columns. This is available to test now from the Test Tool Shed, along with other enhancements like more masking tools and an update to BLAST+ 2.2.29:
http://testtoolshed.g2.bx.psu.edu/view/peterjc/ncbi_blast_plus
...
Some community testing/comments would be appreciated.
I tested it briefly on my test instance and it looks all fine and seems to work :)
Thanks, looking forward to see it in MainTS. Bjoern
Great - thanks. I'm about to update our local production server, and barring any problems will push this BLAST+ update to the main Tool Shed later this week.
This is now live, with a regression about output formats caught and fixed: http://toolshed.g2.bx.psu.edu/view/devteam/ncbi_blast_plus Peter
participants (2)
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Björn Grüning
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Peter Cock