Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
* Access data managers - get data, build indexes, etc
* SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases
* SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database
* BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder
* BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder
* Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching
* View managed data by manager
* SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases
* SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database
* BWA indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1 - builder
* BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder
* Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching
* View managed data by Tool Data Table
* snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases
* snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb
* snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb
* snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations
* bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes
* bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color
* all_fastahttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta * * * * ________________________________________ * From: Björn Grüning [bjoern.gruening@gmail.com] * Sent: Wednesday, April 02, 2014 12:55 PM * To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu * Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * * Hi, * * that means that the snpeff annotation is missing. You can install it * with the snpeff datamanager under your Admin panel. Have a look at the * wiki page about data-manager and the snpeff wrapper documentation. * You do not need to edit xml files. If so that is an bug in the wrapper * and we should fix it :) * * Cheers, * Bjoern * * Am 02.04.2014 19:34, schrieb Wang, Xiaofei: * >>> When I used SnpEff in local galaxy instance, I got an error: * >>> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > * > * > * >> Which Java version do you use. Please make sure you are running Java 1.7. * > * > * > * > * > Yes, it is figured, when I changed the java version to 1.7. Thank you so much! * > * > * > * > * > But, I got another error for snpEff. * > * > * > * > * > java.lang.RuntimeException: Property: 'dm5.30.genome' not found * > at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) * > * > * > * > * > * > * > * > * > What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply. * > * > * > * > * > Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? ) * > * > * > * > * > <param name="genomeVersion" type="select" label="Genome"> * > <option value="hg37">Human (hg37)<option> * > <option value="mm37.61">Mouse (mm37.61)<option> * > <param> * > * > * > * > ________________________________________ * > From: Björn Grüning [bjoern.gruening@gmail.com] * > Sent: Wednesday, April 02, 2014 10:35 AM * > To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu * > Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * > * > Hi Xiaofei, * > * >> Dear there, * >> * >> When I used SnpEff in local galaxy instance, I got an error: * >> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > Which Java version do you use. Please make sure you are running Java 1.7. * > * >> Also, when I used the FASTQ Summary Statistics, I got an error like this: * >> Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/... * > * > Can you give us the complete error message. The important part is missing. * > * > Ciao, * > Bjoern * > * >> I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! * >> * >> * >> * >> * >> ___________________________________________________________ * >> Please keep all replies on the list by using "reply all" * >> in your mail client. To manage your subscriptions to this * >> and other Galaxy lists, please use the interface at: * >> http://lists.bx.psu.edu/ * >> * >> To search Galaxy mailing lists use the unified search at: * >> http://galaxyproject.org/search/mailinglists/ * >> * > * > * *
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
Access data managers - get data, build indexes, etc
SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database
BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder
BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by manager
SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database
BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by Tool Data Table
snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases
snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb
snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb
snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations
bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes
bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color
all_fastahttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 12:55 PM
To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi,
that means that the snpeff annotation is missing. You can install it
with the snpeff datamanager under your Admin panel. Have a look at the
wiki page about data-manager and the snpeff wrapper documentation.
You do not need to edit xml files. If so that is an bug in the wrapper
and we should fix it :)
Cheers,
Bjoern
Am 02.04.2014 19:34, schrieb Wang, Xiaofei:
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Yes, it is figured, when I changed the java version to 1.7. Thank you so much!
But, I got another error for snpEff.
java.lang.RuntimeException: Property: 'dm5.30.genome' not found
at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92)at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481)at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444)at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345)at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply.
Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? )
<param name="genomeVersion" type="select" label="Genome">
<option value="hg37">Human (hg37)<option><option value="mm37.61">Mouse (mm37.61)<option><param>
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 10:35 AM
To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear there,
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Also, when I used the FASTQ Summary Statistics, I got an error like this:
Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/...
Can you give us the complete error message. The important part is missing.
Ciao,
Bjoern
I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help!
Please keep all replies on the list by using "reply all"
in your mail client. To manage your subscriptions to this
and other Galaxy lists, please use the interface at:
http://lists.bx.psu.edu/To search Galaxy mailing lists use the unified search at:
http://galaxyproject.org/search/mailinglists/
After SnpEff Databases > Execute you still need to pick genome of
interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
You mean "SnpEff Download" under Data Manager? But, there is no response when I click on it.
Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin > Data > Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why?
When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time?
Thank you so much!
Best,
Xiaofei
________________________________________ From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
Access data managers - get data, build indexes, etc
SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database
BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder
BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by manager
SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database
BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by Tool Data Table
snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases
snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb
snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb
snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations
bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes
bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color
all_fastahttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 12:55 PM
To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi,
that means that the snpeff annotation is missing. You can install it
with the snpeff datamanager under your Admin panel. Have a look at the
wiki page about data-manager and the snpeff wrapper documentation.
You do not need to edit xml files. If so that is an bug in the wrapper
and we should fix it :)
Cheers,
Bjoern
Am 02.04.2014 19:34, schrieb Wang, Xiaofei:
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Yes, it is figured, when I changed the java version to 1.7. Thank you so much!
But, I got another error for snpEff.
java.lang.RuntimeException: Property: 'dm5.30.genome' not found
at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92)at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481)at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444)at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345)at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320)at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply.
Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? )
<param name="genomeVersion" type="select" label="Genome">
<option value="hg37">Human (hg37)<option><option value="mm37.61">Mouse (mm37.61)<option><param>
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 10:35 AM
To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear there,
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Also, when I used the FASTQ Summary Statistics, I got an error like this:
Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/...
Can you give us the complete error message. The important part is missing.
Ciao,
Bjoern
I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help!
Please keep all replies on the list by using "reply all"
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and other Galaxy lists, please use the interface at:
http://lists.bx.psu.edu/To search Galaxy mailing lists use the unified search at:
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Am 03.04.2014 04:34, schrieb Wang, Xiaofei:
After SnpEff Databases > Execute you still need to pick genome of
interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
You mean "SnpEff Download" under Data Manager? But, there is no response when I click on it.
It's a little bit complicated. If you click on the "SnpEff Download" under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool "SnpEff Databases" there you can choose which genome annotation you want to install. To sum it up.
1. click "SnpEff Download" 2. choose one genome from "SnpEff Databases" and install it
Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin > Data > Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why?
Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel?
Cheers, Bjoern
When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time?
Thank you so much!
Best,
Xiaofei
From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
* Access data managers - get data, build indexes, etc * SnpEff Databases<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4> - Read the list of available snpEff databases * SnpEff Download<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4> - Download a new database * BWA index<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1> - builder * BWA Color index<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1> - builder * Reference Genome<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1> - fetching * View managed data by manager * SnpEff Databases<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1> - Read the list of available snpEff databases * SnpEff Download<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1> - Download a new database * BWA index<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1> - builder * BWA Color index<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1> - builder * Reference Genome<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1> - fetching * View managed data by Tool Data Table * snpeff_databases<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases> * snpeff_genomedb<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb> * snpeff_regulationdb<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb> * snpeff_annotations<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations> * bwa_indexes<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes> * bwa_indexes_color<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color> * all_fasta<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta> * * * * ________________________________________ * From: Björn Grüning [bjoern.gruening@gmail.com] * Sent: Wednesday, April 02, 2014 12:55 PM * To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu * Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * * Hi, * * that means that the snpeff annotation is missing. You can install it * with the snpeff datamanager under your Admin panel. Have a look at the * wiki page about data-manager and the snpeff wrapper documentation. * You do not need to edit xml files. If so that is an bug in the wrapper * and we should fix it :) * * Cheers, * Bjoern * * Am 02.04.2014 19:34, schrieb Wang, Xiaofei: * >>> When I used SnpEff in local galaxy instance, I got an error: * >>> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > * > * > * >> Which Java version do you use. Please make sure you are running Java 1.7. * > * > * > * > * > Yes, it is figured, when I changed the java version to 1.7. Thank you so much! * > * > * > * > * > But, I got another error for snpEff. * > * > * > * > * > java.lang.RuntimeException: Property: 'dm5.30.genome' not found * > at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) * > * > * > * > * > * > * > * > * > What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply. * > * > * > * > * > Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? ) * > * > * > * > * > <param name="genomeVersion" type="select" label="Genome"> * > <option value="hg37">Human (hg37)<option> * > <option value="mm37.61">Mouse (mm37.61)<option> * > <param> * > * > * > * > ________________________________________ * > From: Björn Grüning [bjoern.gruening@gmail.com] * > Sent: Wednesday, April 02, 2014 10:35 AM * > To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu * > Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * > * > Hi Xiaofei, * > * >> Dear there, * >> * >> When I used SnpEff in local galaxy instance, I got an error: * >> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > Which Java version do you use. Please make sure you are running Java 1.7. * > * >> Also, when I used the FASTQ Summary Statistics, I got an error like this: * >> Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/... * > * > Can you give us the complete error message. The important part is missing. * > * > Ciao, * > Bjoern * > * >> I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! * >> * >> * >> * >> * >> ___________________________________________________________ * >> Please keep all replies on the list by using "reply all" * >> in your mail client. To manage your subscriptions to this * >> and other Galaxy lists, please use the interface at: * >> http://lists.bx.psu.edu/ * >> * >> To search Galaxy mailing lists use the unified search at: * >> http://galaxyproject.org/search/mailinglists/ * >> * > * > * *
- click "SnpEff Download"
- choose one genome from "SnpEff Databases" and install it
Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is:
java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42
at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)
I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem?
May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times):
Data Manager: snpeff_genomedb value name path dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data
________________________________________ From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Am 03.04.2014 04:34, schrieb Wang, Xiaofei:
After SnpEff Databases > Execute you still need to pick genome of
interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
You mean "SnpEff Download" under Data Manager? But, there is no response when I click on it.
It's a little bit complicated. If you click on the "SnpEff Download" under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool "SnpEff Databases" there you can choose which genome annotation you want to install. To sum it up.
1. click "SnpEff Download" 2. choose one genome from "SnpEff Databases" and install it
Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin > Data > Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why?
Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel?
Cheers, Bjoern
When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time?
Thank you so much!
Best,
Xiaofei
From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
* Access data managers - get data, build indexes, etc * SnpEff Databases<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4> - Read the list of available snpEff databases * SnpEff Download<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4> - Download a new database * BWA index<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1> - builder * BWA Color index<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1> - builder * Reference Genome<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1> - fetching * View managed data by manager * SnpEff Databases<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1> - Read the list of available snpEff databases * SnpEff Download<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1> - Download a new database * BWA index<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1> - builder * BWA Color index<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1> - builder * Reference Genome<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1> - fetching * View managed data by Tool Data Table * snpeff_databases<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases> * snpeff_genomedb<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb> * snpeff_regulationdb<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb> * snpeff_annotations<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations> * bwa_indexes<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes> * bwa_indexes_color<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color> * all_fasta<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta> * * * * ________________________________________ * From: Björn Grüning [bjoern.gruening@gmail.com] * Sent: Wednesday, April 02, 2014 12:55 PM * To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu * Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * * Hi, * * that means that the snpeff annotation is missing. You can install it * with the snpeff datamanager under your Admin panel. Have a look at the * wiki page about data-manager and the snpeff wrapper documentation. * You do not need to edit xml files. If so that is an bug in the wrapper * and we should fix it :) * * Cheers, * Bjoern * * Am 02.04.2014 19:34, schrieb Wang, Xiaofei: * >>> When I used SnpEff in local galaxy instance, I got an error: * >>> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > * > * > * >> Which Java version do you use. Please make sure you are running Java 1.7. * > * > * > * > * > Yes, it is figured, when I changed the java version to 1.7. Thank you so much! * > * > * > * > * > But, I got another error for snpEff. * > * > * > * > * > java.lang.RuntimeException: Property: 'dm5.30.genome' not found * > at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) * > * > * > * > * > * > * > * > * > What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply. * > * > * > * > * > Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? ) * > * > * > * > * > <param name="genomeVersion" type="select" label="Genome"> * > <option value="hg37">Human (hg37)<option> * > <option value="mm37.61">Mouse (mm37.61)<option> * > <param> * > * > * > * > ________________________________________ * > From: Björn Grüning [bjoern.gruening@gmail.com] * > Sent: Wednesday, April 02, 2014 10:35 AM * > To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu * > Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * > * > Hi Xiaofei, * > * >> Dear there, * >> * >> When I used SnpEff in local galaxy instance, I got an error: * >> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > Which Java version do you use. Please make sure you are running Java 1.7. * > * >> Also, when I used the FASTQ Summary Statistics, I got an error like this: * >> Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/... * > * > Can you give us the complete error message. The important part is missing. * > * > Ciao, * > Bjoern * > * >> I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! * >> * >> * >> * >> * >> ___________________________________________________________ * >> Please keep all replies on the list by using "reply all" * >> in your mail client. To manage your subscriptions to this * >> and other Galaxy lists, please use the interface at: * >> http://lists.bx.psu.edu/ * >> * >> To search Galaxy mailing lists use the unified search at: * >> http://galaxyproject.org/search/mailinglists/ * >> * > * > * *
Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download the database by command again: java -jar snpEff.jar download dm5.42
Thank you so much!
Best,
Xiaofei _____________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
- click "SnpEff Download"
- choose one genome from "SnpEff Databases" and install it
Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is:
java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42
at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)
I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem?
May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times):
Data Manager: snpeff_genomedb value name path dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data
________________________________________ From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Am 03.04.2014 04:34, schrieb Wang, Xiaofei:
After SnpEff Databases > Execute you still need to pick genome of
interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
You mean "SnpEff Download" under Data Manager? But, there is no response when I click on it.
It's a little bit complicated. If you click on the "SnpEff Download" under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool "SnpEff Databases" there you can choose which genome annotation you want to install. To sum it up.
1. click "SnpEff Download" 2. choose one genome from "SnpEff Databases" and install it
Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin > Data > Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why?
Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel?
Cheers, Bjoern
When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time?
Thank you so much!
Best,
Xiaofei
From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
* Access data managers - get data, build indexes, etc * SnpEff Databases<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4> - Read the list of available snpEff databases * SnpEff Download<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4> - Download a new database * BWA index<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1> - builder * BWA Color index<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1> - builder * Reference Genome<http://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1> - fetching * View managed data by manager * SnpEff Databases<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1> - Read the list of available snpEff databases * SnpEff Download<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1> - Download a new database * BWA index<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1> - builder * BWA Color index<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1> - builder * Reference Genome<http://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1> - fetching * View managed data by Tool Data Table * snpeff_databases<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases> * snpeff_genomedb<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb> * snpeff_regulationdb<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb> * snpeff_annotations<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations> * bwa_indexes<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes> * bwa_indexes_color<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color> * all_fasta<http://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta> * * * * ________________________________________ * From: Björn Grüning [bjoern.gruening@gmail.com] * Sent: Wednesday, April 02, 2014 12:55 PM * To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu * Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * * Hi, * * that means that the snpeff annotation is missing. You can install it * with the snpeff datamanager under your Admin panel. Have a look at the * wiki page about data-manager and the snpeff wrapper documentation. * You do not need to edit xml files. If so that is an bug in the wrapper * and we should fix it :) * * Cheers, * Bjoern * * Am 02.04.2014 19:34, schrieb Wang, Xiaofei: * >>> When I used SnpEff in local galaxy instance, I got an error: * >>> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > * > * > * >> Which Java version do you use. Please make sure you are running Java 1.7. * > * > * > * > * > Yes, it is figured, when I changed the java version to 1.7. Thank you so much! * > * > * > * > * > But, I got another error for snpEff. * > * > * > * > * > java.lang.RuntimeException: Property: 'dm5.30.genome' not found * > at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345) * > at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) * > at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) * > * > * > * > * > * > * > * > * > What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply. * > * > * > * > * > Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? ) * > * > * > * > * > <param name="genomeVersion" type="select" label="Genome"> * > <option value="hg37">Human (hg37)<option> * > <option value="mm37.61">Mouse (mm37.61)<option> * > <param> * > * > * > * > ________________________________________ * > From: Björn Grüning [bjoern.gruening@gmail.com] * > Sent: Wednesday, April 02, 2014 10:35 AM * > To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu * > Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * > * > Hi Xiaofei, * > * >> Dear there, * >> * >> When I used SnpEff in local galaxy instance, I got an error: * >> Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * > * > Which Java version do you use. Please make sure you are running Java 1.7. * > * >> Also, when I used the FASTQ Summary Statistics, I got an error like this: * >> Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/... * > * > Can you give us the complete error message. The important part is missing. * > * > Ciao, * > Bjoern * > * >> I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! * >> * >> * >> * >> * >> ___________________________________________________________ * >> Please keep all replies on the list by using "reply all" * >> in your mail client. To manage your subscriptions to this * >> and other Galaxy lists, please use the interface at: * >> http://lists.bx.psu.edu/ * >> * >> To search Galaxy mailing lists use the unified search at: * >> http://galaxyproject.org/search/mailinglists/ * >> * > * > * *
___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
I am sorry to bother again about this problem.
I would like to use Drosophila melanogaster : BDGP5.74. I did the two steps you suggested to me to install the annotation. I also did from command line. But, there is no the option of BDGP5.74 from the "Genome" drop-down menu in SnpEff. The version of SnpEff is version 1.0 and I used SnpEfff in CloudMap workflow. Do you know why and how to figure it out? (In fact, it works fine for BDGP5.66.)
Thanks
Best,
Xiaofei ________________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Thursday, April 03, 2014 3:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download the database by command again: java -jar snpEff.jar download dm5.42
Thank you so much!
Best,
Xiaofei _____________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
- click "SnpEff Download"
- choose one genome from "SnpEff Databases" and install it
Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is:
java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42
at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)
I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem?
May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times):
Data Manager: snpeff_genomedb value name path dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data
________________________________________ From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Am 03.04.2014 04:34, schrieb Wang, Xiaofei:
After SnpEff Databases > Execute you still need to pick genome of
interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
You mean "SnpEff Download" under Data Manager? But, there is no response when I click on it.
It's a little bit complicated. If you click on the "SnpEff Download" under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool "SnpEff Databases" there you can choose which genome annotation you want to install. To sum it up.
1. click "SnpEff Download" 2. choose one genome from "SnpEff Databases" and install it
Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin > Data > Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why?
Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel?
Cheers, Bjoern
When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time?
Thank you so much!
Best,
Xiaofei
From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
Access data managers - get data, build indexes, etc
SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database
BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder
BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by manager
SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database
BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by Tool Data Table
snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases
snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb
snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb
snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations
bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes
bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color
all_fastahttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 12:55 PM
To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi,
that means that the snpeff annotation is missing. You can install it
with the snpeff datamanager under your Admin panel. Have a look at the
wiki page about data-manager and the snpeff wrapper documentation.
You do not need to edit xml files. If so that is an bug in the wrapper
and we should fix it :)
Cheers,
Bjoern
Am 02.04.2014 19:34, schrieb Wang, Xiaofei:
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Yes, it is figured, when I changed the java version to 1.7. Thank you so much!
But, I got another error for snpEff.
java.lang.RuntimeException: Property: 'dm5.30.genome' not found
at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92)
at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481)
at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444)
at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345)
at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)
What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply.
Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? )
<param name="genomeVersion" type="select" label="Genome">
<option value="hg37">Human (hg37)<option>
<option value="mm37.61">Mouse (mm37.61)<option>
<param>
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 10:35 AM
To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear there,
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Also, when I used the FASTQ Summary Statistics, I got an error like this:
Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/...
Can you give us the complete error message. The important part is missing.
Ciao,
Bjoern
I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help!
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Dear there,
Could someone tell me why there is no Drosophila melanogaster : BDGP 5.74 in the drop-down menu of SnpEff for local Galaxy?
In fact, I could find it (BDGP 5.74) in SnpEff Databases and also download it from SnpEff Download (Data manager: Admin > Manage local data (beta)). But, I still can not find it in the menu of SnpEff after downloading and installing it by the "two-steps" as below.
Thanks a lot!
Best,
Xiaofei ________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Friday, April 11, 2014 5:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
I am sorry to bother again about this problem.
I would like to use Drosophila melanogaster : BDGP5.74. I did the two steps you suggested to me to install the annotation. I also did from command line. But, there is no the option of BDGP5.74 from the "Genome" drop-down menu in SnpEff. The version of SnpEff is version 1.0 and I used SnpEfff in CloudMap workflow. Do you know why and how to figure it out? (In fact, it works fine for BDGP5.66.)
Thanks
Best,
Xiaofei ________________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Thursday, April 03, 2014 3:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download the database by command again: java -jar snpEff.jar download dm5.42
Thank you so much!
Best,
Xiaofei _____________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
- click "SnpEff Download"
- choose one genome from "SnpEff Databases" and install it
Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is:
java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42
at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)
I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem?
May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times):
Data Manager: snpeff_genomedb value name path dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data
________________________________________ From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Am 03.04.2014 04:34, schrieb Wang, Xiaofei:
After SnpEff Databases > Execute you still need to pick genome of
interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
You mean "SnpEff Download" under Data Manager? But, there is no response when I click on it.
It's a little bit complicated. If you click on the "SnpEff Download" under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool "SnpEff Databases" there you can choose which genome annotation you want to install. To sum it up.
1. click "SnpEff Download" 2. choose one genome from "SnpEff Databases" and install it
Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin > Data > Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why?
Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel?
Cheers, Bjoern
When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time?
Thank you so much!
Best,
Xiaofei
From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
Access data managers - get data, build indexes, etc
SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database
BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder
BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by manager
SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database
BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by Tool Data Table
snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases
snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb
snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb
snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations
bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes
bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color
all_fastahttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 12:55 PM
To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi,
that means that the snpeff annotation is missing. You can install it
with the snpeff datamanager under your Admin panel. Have a look at the
wiki page about data-manager and the snpeff wrapper documentation.
You do not need to edit xml files. If so that is an bug in the wrapper
and we should fix it :)
Cheers,
Bjoern
Am 02.04.2014 19:34, schrieb Wang, Xiaofei:
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Yes, it is figured, when I changed the java version to 1.7. Thank you so much!
But, I got another error for snpEff.
java.lang.RuntimeException: Property: 'dm5.30.genome' not found
at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92)
at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481)
at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444)
at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345)
at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)
What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply.
Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? )
<param name="genomeVersion" type="select" label="Genome">
<option value="hg37">Human (hg37)<option>
<option value="mm37.61">Mouse (mm37.61)<option>
<param>
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 10:35 AM
To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear there,
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Also, when I used the FASTQ Summary Statistics, I got an error like this:
Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/...
Can you give us the complete error message. The important part is missing.
Ciao,
Bjoern
I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help!
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Dear folks,
Why could not I find BDGP 5.74 in the drop-down menu of SnpEff? If I want to use this version, what should I do or what other steps do I need to install it?
Thank you so much!
________________________________ From: Wang, Xiaofei Sent: Monday, April 14, 2014 9:50 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: RE: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Dear there,
Could someone tell me why there is no Drosophila melanogaster : BDGP 5.74 in the drop-down menu of SnpEff for local Galaxy?
In fact, I could find it (BDGP 5.74) in SnpEff Databases and also download it from SnpEff Download (Data manager: Admin > Manage local data (beta)). But, I still can not find it in the menu of SnpEff after downloading and installing it by the "two-steps" as below.
Thanks a lot!
Best,
Xiaofei ________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Friday, April 11, 2014 5:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
I am sorry to bother again about this problem.
I would like to use Drosophila melanogaster : BDGP5.74. I did the two steps you suggested to me to install the annotation. I also did from command line. But, there is no the option of BDGP5.74 from the "Genome" drop-down menu in SnpEff. The version of SnpEff is version 1.0 and I used SnpEfff in CloudMap workflow. Do you know why and how to figure it out? (In fact, it works fine for BDGP5.66.)
Thanks
Best,
Xiaofei ________________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Thursday, April 03, 2014 3:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download the database by command again: java -jar snpEff.jar download dm5.42
Thank you so much!
Best,
Xiaofei _____________________________________ From: galaxy-dev-bounces@lists.bx.psu.edu [galaxy-dev-bounces@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfwang@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
- click "SnpEff Download"
- choose one genome from "SnpEff Databases" and install it
Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is:
java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42
at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)
I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem?
May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times):
Data Manager: snpeff_genomedb value name path dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data
________________________________________ From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Am 03.04.2014 04:34, schrieb Wang, Xiaofei:
After SnpEff Databases > Execute you still need to pick genome of
interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
You mean "SnpEff Download" under Data Manager? But, there is no response when I click on it.
It's a little bit complicated. If you click on the "SnpEff Download" under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool "SnpEff Databases" there you can choose which genome annotation you want to install. To sum it up.
1. click "SnpEff Download" 2. choose one genome from "SnpEff Databases" and install it
Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin > Data > Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why?
Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel?
Cheers, Bjoern
When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time?
Thank you so much!
Best,
Xiaofei
From: Björn Grüning [bjoern.gruening@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin > Data > Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases > Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty.
After SnpEff Databases > Execute you still need to pick genome of interest and run "SnpEff Download". SnpEff Databases just downloads a list of available genomes.
Hope that will help! Bjoern
Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel?
I really appreciate for your help, and thank you so much for your patience!
Best,
Xiaofei Data Manager
Choose your data managing option from below.
Access data managers - get data, build indexes, etc
SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database
BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder
BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by manager
SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases
SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database
BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder
Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching
View managed data by Tool Data Table
snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases
snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb
snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb
snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations
bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes
bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color
all_fastahttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 12:55 PM
To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi,
that means that the snpeff annotation is missing. You can install it
with the snpeff datamanager under your Admin panel. Have a look at the
wiki page about data-manager and the snpeff wrapper documentation.
You do not need to edit xml files. If so that is an bug in the wrapper
and we should fix it :)
Cheers,
Bjoern
Am 02.04.2014 19:34, schrieb Wang, Xiaofei:
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Yes, it is figured, when I changed the java version to 1.7. Thank you so much!
But, I got another error for snpEff.
java.lang.RuntimeException: Property: 'dm5.30.genome' not found
at ca.mcgill.mcb.pcingola.interval.Genome.<init>(Genome.java:92)
at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481)
at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444)
at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345)
at ca.mcgill.mcb.pcingola.snpEffect.Config.<init>(Config.java:96)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320)
at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83)
What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply.
Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? )
<param name="genomeVersion" type="select" label="Genome">
<option value="hg37">Human (hg37)<option>
<option value="mm37.61">Mouse (mm37.61)<option>
<param>
From: Björn Grüning [bjoern.gruening@gmail.com]
Sent: Wednesday, April 02, 2014 10:35 AM
To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu
Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei,
Dear there,
When I used SnpEff in local galaxy instance, I got an error:
Exception in thread "main" java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j
Which Java version do you use. Please make sure you are running Java 1.7.
Also, when I used the FASTQ Summary Statistics, I got an error like this:
Traceback (most recent call last): File "/Users/...../softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py", line 48, in if __name__ == "__main__": main() File "/Users/...
Can you give us the complete error message. The important part is missing.
Ciao,
Bjoern
I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help!
Please keep all replies on the list by using "reply all"
in your mail client. To manage your subscriptions to this
and other Galaxy lists, please use the interface at:
To search Galaxy mailing lists use the unified search at:
___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
galaxy-dev@lists.galaxyproject.org
-
Björn Grüning -
Wang, Xiaofei