Error running bedgraph to bigwig on local galaxy
When attempting to generate a bigwig file I receive this error. hashMustFindVal: 'GL000192.1' not found Fatal error: Matched on Error Error running wigToBigWig. I have added the chrom len files for galaxy and update the location path but an unsure what could be causing this issue.
Are you using the chromosome lengths for the correct organism and source? GL000192.1 is a contig name used by Ensembl and Gencode (but not UCSC!) for GRCh37 (aka hg19). Devon -- Devon Ryan, Ph.D. Email: dpryan@dpryan.com Data Manager/Bioinformatician Max Planck Institute of Immunobiology and Epigenetics Stübeweg 51 79108 Freiburg Germany On Wed, Nov 23, 2016 at 4:12 PM, evan clark <eclark28@fau.edu> wrote:
When attempting to generate a bigwig file I receive this error.
hashMustFindVal: 'GL000192.1' not found Fatal error: Matched on Error Error running wigToBigWig.
I have added the chrom len files for galaxy and update the location path but an unsure what could be causing this issue.
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Yes, these alignments were done to ensemble, not UCSC. ---Regards, Evan Clark Reminder: If this message is requesting you to verify your account or provides links for you to login to your account, you are advised to ignore such requests. Neither FAU nor legitimate financial institutions will ever ask you for this information via email or via a link in an email. Are you using the chromosome lengths for the correct organism and source? GL000192.1 is a contig name used by Ensembl and Gencode (but not UCSC!) for GRCh37 (aka hg19). Devon -- Devon Ryan, Ph.D. Email: dpryan@dpryan.com Data Manager/Bioinformatician Max Planck Institute of Immunobiology and Epigenetics Stübeweg 51 79108 Freiburg Germany On Wed, Nov 23, 2016 at 4:12 PM, evan clark <eclark28@fau.edu> wrote:
When attempting to generate a bigwig file I receive this error.
hashMustFindVal: 'GL000192.1' not found Fatal error: Matched on Error Error running wigToBigWig.
I have added the chrom len files for galaxy and update the location path but an unsure what could be causing this issue.
___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: https://lists.galaxyproject.org/
To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
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evan clark