My installation of the fastx toolkit seems to be having quite a few problems. I can run the groomer on my data, although it seems to remove a lot more data than expected. I often end up with empty files. More concerning right now is the attempt to calculate quality statistics. These never work for me, although I've been able to get it to work on the main PSU galaxy instance with the same data. Command line usage fails with the same error: fastx_quality_stats: Invalid quality score value (char '%' ord 37 quality value -27) on line 4 * * *The error may be misleading, but its all I can gather from any out put right now. It may be more appropriate to ask this to someone in the FASTX community, but figured I'd shoot here as well, in case this has come up before. * * * *Thanks!* * * *Juan Perin* * *